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DIF001

Sigma-Aldrich

3T3-L1 DIFFERENTIATION KIT

Sufficient for 100 mL differentiation medium

Synonyme(s) :

3T3-L1 Differentiation Kit

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Forme

solution

Utilisation

100 mL differentiation medium

Technique(s)

cell culture | mammalian: suitable

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. The 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes.

Application

3T3-L1 DIFFERENTIATION KIT has been used in:
  • Differentiation of 3T3-L1 preadipocytes to adipocytes.
  • Study of obesity, adipogenesis, lipolysis and lipid metabolism
  • Differentiation of 3T3-L1 preadipocytes to adipocytes
  • Study of obesity, adipogenesis, lipolysis and lipid metabolism.

Composants

Insulin (1.5 mg/ml) 0.6 ml (Catalog Number DIF001A, Green Cap)
Differentiation Cocktail, 1000x (lyophilized) 1 vl (Catalog Number DIF001B, Yellow Cap)
DMSO (anhydrous) 0.5 ml (Catalog Number DIF001C, Blue Cap)

Stockage et stabilité

Store the kit at −20 °C, protected from light.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H360FD

Classification des risques

Repr. 1B

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Consulter la Bibliothèque de documents

Jérémie Gautheron et al.
Nature communications, 7, 11869-11869 (2016-06-22)
Receptor-interacting protein kinase 3 (RIPK3) mediates necroptosis, a form of programmed cell death that promotes inflammation in various pathological conditions, suggesting that it might be a privileged pharmacological target. However, its function in glucose homeostasis and obesity has been unknown.
Fuu-Jen Tsai et al.
Frontiers in pharmacology, 9, 1004-1004 (2018-09-21)
Antiretroviral (ART) drugs has previously been associated with lipodystrophic syndrome, metabolic consequences, and neuropsychiatric complications. ART drugs include three main classes of protease inhibitors (PIs), nucleoside analog reverse transcriptase inhibitors (NRTIs), and non-nucleoside reverse transcriptase inhibitors (NNRTIs). Our previous work
Tamar Golan et al.
Science signaling, 12(591) (2019-07-25)
Transforming growth factor-β (TGF-β) superfamily members are critical signals in tissue homeostasis and pathogenesis. Melanoma grows in the epidermis and invades the dermis before metastasizing. This disease progression is accompanied by increased sensitivity to microenvironmental TGF-β. Here, we found that
Ke Song et al.
JCI insight, 5(21) (2020-10-02)
Chronic kidney disease (CKD) induces the failure of arteriovenous fistulas (AVFs) and promotes the differentiation of vascular adventitial GLI1-positive mesenchymal stem cells (GMCs). However, the roles of GMCs in forming neointima in AVFs remain unknown. GMCs isolated from CKD mice

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