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D8312

Sigma-Aldrich

REDTaq® Genomic DNA Polymerase

with MgCl2

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About This Item

Numéro MDL:
MFCD01635818
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.55

Forme

liquid

Utilisation

sufficient for 1000 reactions
 sufficient for 250 reactions
 sufficient for 2500 reactions

Caractéristiques

Difficult Templates/Specialty Enzymes PCR
dNTPs included: no
hotstart: no

Concentration

1 unit/μL

Technique(s)

PCR: suitable

Couleur

red

Entrée

purified DNA

Application(s)

agriculture

Conditions d'expédition

wet ice

Température de stockage

−20°C

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Description générale

REDTaq® Genomic DNA Polymerase is a unique blend of Taq DNA Polymerase with an inert red dye. This special formulation is designed to provide enhanced amplification of more complex or genomic templates. REDTaq Genomic DNA Polymerase is highly sensitive, produces increased yields and is capable of generating longer product lengths. It has all the advantages of REDTaq DNA polymerase, such as easy visualization of enzyme addition and complete reaction mixing, and direct loading to an agarose gel. The inert red dye does not effect automated or manual sequencing, restriction digestions or other downstream applications. The dye can easily removed by any standard purification method.
The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides (the building blocks of DNA). These enzymes are essential for DNA replication and usually work in pairs. It creates two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.

Application

REDTaq® Genomic DNA Polymerase is used for routine PCR amplification of genomic DNA.

Caractéristiques et avantages

  • Enhanced amplification on genomic and difficult DNA templates
  • Same great performance as Taq DNA Polymerase in a more convenient format for high throughput applications
  • Quick recognition and confirmation of appropriate mixing
  • No loading buffers or tracking dyes necessary. Sample can be taken directly from reaction and loaded onto an agarose gel
  • The samples can be re-amplified as in nested PCR
  • The red dye migrates faster than bromophenol blue
  • Greater consistency across reactions due to proper mixing

Définition de l'unité

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74°C.

Informations légales

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

J A F Demandt et al.
Scientific reports, 11(1), 425-425 (2021-01-13)
Hypoxia is prevalent in atherosclerotic plaques, promoting plaque aggravation and subsequent cardiovascular disease (CVD). Transmembrane protein carbonic anhydrase IX (CAIX) is hypoxia-induced and can be shed into the circulation as soluble CAIX (sCAIX). As plaque macrophages are hypoxic, we hypothesized a
Preparation of genomic DNA from Dictyostelium discoideum for PCR analysis.
Steve J Charette et al.
BioTechniques, 36(4), 574-575 (2004-04-20)
L M Winton et al.
Phytopathology, 92(1), 112-116 (2008-10-24)
ABSTRACT Phaeocryptopus gaeumannii is a widespread foliar parasite of Douglas-fir. Although normally innocuous, the fungus also causes the defoliating disease Swiss needle cast in heavily infected needles. The extent of P. gaeumannii colonization in Douglas-fir foliage was estimated with real-time
Genotyping of HLA-B27 by real-time PCR without hybridization probes.
M A Bon et al.
Clinical chemistry, 46(7), 1000-1002 (2000-07-15)
Gregory G Martin et al.
The Journal of biological chemistry, 278(24), 21429-21438 (2003-04-03)
Although liver fatty acid-binding protein (L-FABP) is an important binding site for various hydrophobic ligands in hepatocytes, its in vivo significance is not understood. We have therefore created L-FABP null mice and report here their initial analysis, focusing on the
Afficher tous les articles scientifiques apparentés

Protocoles

Applications with REDTaq™

Reviews the applications and benefits for RedTaq, including standard RedTaq, Hot Start RedTaq and RedTaq for genomic DNA PCR.

Amplification of Genomic DNA using REDTaq® DNA Polymerase

Reactions using REDTaq® DNA polymerase are formulated as any PCR mixtures. There are no additional reaction preparation steps or protocol changes required.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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