CYTOCOX1
Cytochrome c Oxidase Assay Kit
sufficient for 100 tests, soluble and membrane bound mitochondria
Synonyme(s) :
Cytochrome c Oxidase Activity Assay Kit
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About This Item
Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84
Produits recommandés
Niveau de qualité
Utilisation
sufficient for 100 tests
Conditions d'expédition
dry ice
Température de stockage
−20°C
Informations sur le gène
human ... COX1(4512)
Application
The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.
Actions biochimiques/physiologiques
Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.
Caractéristiques et avantages
- Simple, optimized protocol - Obtain reproducible results without special training needs
- Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
- Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
- May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
- Use to analyze the intactness of mitochondrial membranes
- Simple colorimetric measurement of solution
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Dam. 1 - Skin Irrit. 2
Code de la classe de stockage
10 - Combustible liquids
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