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CS0010

Sigma-Aldrich

β-Secretase (BACE1) Activity Detection Kit (Fluorescent)

1 kit sufficient for 250 reactions

Synonyme(s) :

BACE1 Activity Detection Kit (Fluorescent)

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Niveau de qualité

300

Utilisation

 kit sufficient for 250 reactions

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

human ... BACE1(23621)

Actions biochimiques/physiologiques

BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery.

Adéquation

The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1.

Composants de kit seuls

Réf. du produit
Description
  • Fluorescent Assay Buffer 50 mL
  • Stop Solution 15 mL
  • Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL
  • Assay Standard 140 μL
  • BACE1 (β−Secretase) 300 units 100 μL

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Vijay K Nuthakki et al.
Bioorganic chemistry, 90, 103062-103062 (2019-06-21)
Alkaloids have always been a great source of cholinesterase inhibitors. Numerous studies have shown that inhibiting acetylcholinesterase as well as butyrylcholinetserase is advantageous, and have better chances of success in preclinical/ clinical settings. With the objective to discover dual cholinesterase
Lucas J Gutierrez et al.
Journal of biomolecular structure & dynamics, 35(2), 413-426 (2016-01-28)
We report here two new small-size peptides acting as modulators of the β-site APP cleaving enzyme 1 (BACE1) exosite. Ac-YPYFDPL-NH2 and Ac-YPYDIPL-NH2 displayed a moderate but significant inhibitory effect on BACE1. These peptides were obtained from a molecular modeling study.
L Hong et al.
Biochemical Society transactions, 30(4), 530-534 (2002-08-28)
As beta-secretase, memapsin 2 cleaves amyloid-beta precursor protein, which leads ultimately to the onset of Alzheimer's disease. As such, memapsin 2 is an excellent target of inhibitor drugs for the treatment of this disease. Here we describe the tools for
Changxing Qi et al.
Fitoterapia, 130, 134-139 (2018-08-31)
Terrusnolides A-D (1-4), four butenolides were isolated from an endophytic Aspergillus from Tripterygium wilfordii. The structures of 1-4 were established by comprehensive spectroscopic analyses and electronic circular dichroism (ECD) calculation. It is interesting that 1 was a butenolide derived by
Raúl Horacio Camarillo-López et al.
Molecules (Basel, Switzerland), 25(21) (2020-11-04)
Alzheimer's disease (AD) is a neurodegenerative disease with no cure nowadays; there is no treatment either to prevent or to stop its progression. In vitro studies suggested that tert-butyl-(4-hydroxy-3-((3-(2-methylpiperidin-yl)propyl)carbamoyl)phenyl) carbamate named the M4 compound can act as both β-secretase and
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