CRE BAG2

95082219, mouse unknown/unspecified, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: CRE BAG2, 95082219
• Source biologique: mouse unknown/unspecified
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse NIH 3T3 fibroblast, Moloney murine leukaemia virus transfected

Description de la lignée cellulaire

CRE BAG2 has been established from the NIH 3T3 fibroblast cell line. Cells were transfected with Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions. CRE BAG2 produces BAG ( a β-gal-transducing vector) and can be used for the package of vectors derived from murine leukaemia viruses. The production of recombinant replication-competent helper viruses has never been reported.

Application

Virus-packaging assays, cell-lineage marking systems

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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