KM703

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Rat x Mouse Hybridoma
• Conditionnement: tube of 5 μg 94090613-DNA-5UG; pkg of vial of cells 94090613-1VL
• ImmunoDon: Rat Lou/MN spleen cells
• Myélome: Sp2/0
• Réactivité: Anti-Pgp-1, murine homologue of CD44
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Rat Lou/MN spleen x Mouse Sp2/0

Description de la lignée cellulaire

The hybridoma KM703 was produced by the fusion of Sp2/0 mouse myeloma cells with spleen cells from a Lou/MN rat immunised with the bone marrow derived stromal cell clone, BMS2. The monoclonal antibody recognises an epitope on Pgp-1, the murine homologue of CD44. It prevents the growth of lymphoid or myeloid cells in long term bone marrow cultures.

Immunogène

BUS 2 cells

Application

Determination of ligand specifities and function of Pgp-1/CD44 molecules.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Milieu de culture

RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Autres remarques

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