KYSE-270

94072021, human esophagus, Epithelial

• Synonyme(s) : KY-270 Cells, KYSE270 Cells
• Code UNSPSC : 41106514

Propriétés

• product name: KYSE-270, 94072021
• Source biologique: human esophagus
• Mode de croissance: Adherent
• Caryotype: Hypotriploid with 16% polyploidy
• Morphologie: Epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human oesophageal squamous cell carcinoma

Description de la lignée cellulaire

Established from the well differentiated invasive oesophageal squamous cell carcinoma resected from the middle intra-thoracic oesophagus of a 79-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC.

Milieu de culture

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS)

Procédure de repiquage

Split confluent cultures 1:5 every second day using trypsin/EDTA;5% CO₂; 37°C. Doubling time: 24 hours. Recommended seeding density: 2.5 x 10,000 cells/cm².

Autres remarques

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