Z41

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 93022520, Reactivity: Anti-IgG2a, mouse

• Code UNSPSC : 41106514

Propriétés

• Source biologique: Mouse x Mouse Hybridoma
• Conditionnement: tube of 5 μg 93022520-DNA-5UG; pkg of vial of cells 93022520-1VL
• ImmunoDon: BALB/c mouse spleen
• Myélome: Sp2/0
• Réactivité: Anti-IgG2a, mouse
• Technique(s): cell culture | mammalian: suitable
• Isotype: IgM
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Mouse Sp2/0 x Mouse BALB/c spleen

Description de la lignée cellulaire

The immunisation protocol involved repeated intranasal infection with a single i.v. injection before fusion. The IgM antibodies bind murine IgG2a of "a" allotype and Fc region. There is no cross-reactivity with A/PR/8/34 influenza virus, DNP, NP, DNA or cytoskeletal proteins. Contains a CH2 domain.

Immunogène

A/PR/8/34 mouse pathogenic human influenza virus

Application

Differential detection of complexed IgG2a in the presence of native IgG2a, allotype detection or quantitation.

Milieu de culture

RPMI 1640 or DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS) (can be adapted to 1% Foetal Bovine Serum (FBS))

Procédure de repiquage

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

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