293 N3S

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Code UNSPSC : 41106514

Propriétés

• Source biologique: human kidney
• Conditionnement: tube of 5 μg 92052131-DNA-5UG; pkg of vial of cells 92052131-1VL
• Mode de croissance: Suspension
• Caryotype: Not specified
• Morphologie: Not specified
• Produits: Adenovirus 5 early region (E1) antigens
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human embryo kidney

Description de la lignée cellulaire

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

Profil d'ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Milieu de culture

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

Procédure de repiquage

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

Autres remarques

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