3T3-MSV

90092701, mouse embryo, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: 3T3-MSV, 90092701
• Source biologique: mouse embryo
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Growth factor
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

Product has lost contact inhibition

Origine de la lignée cellulaire

Mouse Swiss 3T3 embryo, Moloney-MSV transformed

Description de la lignée cellulaire

This cell line is of Swiss 3T3 origin, transformed by the Moloney murine sarcoma virus. It is a non-producer of the virus particles. In serum-free media the line will release a growth factor which can be used to stimulate quiescent cells to grow in monolayer as well as in soft agar.

Application

Cell growth studies

Milieu de culture

DMEM + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:6 to 1:8 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Care is required during subculture as cells have a tendency to easily detach from the tissue culture flask.

Autres remarques

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