UKRV-Mel-19b

13012536, human lymph node, Epithelial

• Code UNSPSC : 41106514

Propriétés

• product name: UKRV-Mel-19b, 13012536, from human lymph node
• Source biologique: human lymph node
• Description: Human melanoma, metastasis
• Mode de croissance: Adherent
• Morphologie: Epithelial
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description de la lignée cellulaire

UKRV-Mel-19b (ESTDAB-231) was established from the lymph node metastasis in a 53 year-old female patient with metastatic melanoma (unknown primary stage IV). The UKRV-Mel-19b cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

Profil d'ADN

STR-PCR Data:

Amelogenin: X
CSF1PO: 12
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 11,12
THO1: 9
TPOX: 8
vWA: 14,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) seed new cultures at 1-3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 20 x 10,000 cells/cm² .

Autres remarques

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