Vero/hSLAM

4091501, African green monkey kidney, Fibroblast

• Synonyme(s) : Vero/hSLAM Cells
• Code UNSPSC : 41106514

Propriétés

• product name: Vero/hSLAM, from African green monkey kidney, 04091501, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Source biologique: African green monkey kidney
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: SLAM; also known as CDw150, a receptor for measles virus
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Monkey African Green Kidney human CDw150 (SLAM) mealses virus receptor expression

Description de la lignée cellulaire

Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) , also known as CDw150, which is a receptor for measles virus. Clones of transfected cells were selected in the presence of geneticin (also known as G418). Vero/hSLAM cells can be used to isolate measles virus from human clinical samples and are a substitute for the B95a cell line for this purpose. Mumps susceptibility has also been demonstrated at the depositor′s laboratory.

Milieu de culture

MEM with Earle's salts + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442) + 0.4 mg/ml Geneticin. It is essential that geneticin is added to the culture medium as it is required to maintain the expression of the SLAM gene.

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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