C2230

Coelenterazine, native

solid

• Synonyme(s) : 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one, CLZN
• Formule empirique (notation de Hill): C₂₆H₂₁N₃O₃
• Numéro CAS: 55779-48-1
• Poids moléculaire : 423.46
• Numéro MDL: MFCD00467176
• Code UNSPSC : 12352200
• ID de substance PubChem : 24892477
• Nomenclature NACRES : NA.32

Propriétés

• Essai: ≥95% (TLC)
• Forme: solid
• Solubilité: methanol and ethanol: soluble
• Température de stockage: −20°C
• Chaîne SMILES: Oc1ccc(CC2=NC3=C(Cc4ccccc4)NC(=CN3C2=O)c5ccc(O)cc5)cc1
• InChI: 1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2
• Clé InChI: YHIPILPTUVMWQT-UHFFFAOYSA-N

Description

Description générale

Coelenterazine is a luciferin molecule, which is utilized by several luciferases, such as Renilla, Oplophorus and Periphylla.[1]

Application

Coelenterazine, native was used:-

• For Aequorin assays[2]
• For measuring the intracellular calcium concentration in response to ionomycin or PMA using aequorin in COS-7 cells[3]
• In Bioluminescence imaging for analysing the presence of virus-dependent luciferase activity[4]

Actions biochimiques/physiologiques

Luminophore of the aequorin complex which is oxidized by oxygen to illuminate at 465 nm when Ca²⁺ binds to the complex; used to measure Ca²⁺ concentration in cells with high sensitivity and large dynamic range.

Attention

Protect from exposure to light

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)