C-12375

Human Cardiac Fibroblasts (HCF)

500,000 cryopreserved cells

• Synonyme(s) : HCF cells
• Code UNSPSC : 41106514
• Nomenclature NACRES : NA.81

Propriétés

• Source biologique: human heart (ventricles)
• Conditionnement: pkg of 500,000 cells
• Morphologie: (fibroblast)
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cardiovascular diseases
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Description générale

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Origine de la lignée cellulaire

Heart

Application

Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of the adult heart and are provided in a cryopreserved format. They play a central role in the maintenance of the extracellular matrix in the normal heart and the synthesis of growth factors and cytokines. Under pathophysiological conditions, cardiac fibroblasts are involved in scar formation after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be used to study such processes in vitro.

Qualité

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Avertissement

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Procédure de repiquage

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Autres remarques

Recommended Plating Density: 3500 - 7000 cells per cm²Passage After Thawing: P2Tested Markers: CD90 positive, Smooth muscle alpha-actin negative, slow muscle myosin negativeGuaranteed population doublings: >15

Produits recommandés

Recommended Primary Cell Culture Media:Link

Clause de non-responsabilité

Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable