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APOAC

Sigma-Aldrich

Annexin V-Cy3™ Apoptosis Detection Kit

Synonyme(s) :

Red fluorescent Annexin V apoptosis assay

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 200 tests

Niveau de qualité

200

Conditionnement

pkg of 1 kit

Technique(s)

flow cytometry: suitable

Application(s)

cell analysis
detection

Méthode de détection

fluorometric

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Caractéristiques et avantages

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
  • Rapid labeling of cells. Cell staining takes only 10 minutes.
  • No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
  • 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Principe

Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Informations légales

Cy3 is a trademark of Cytiva

Composants de kit seuls

Réf. du produit
Description
  • Annexin V Cy3.18 Conjugate from human placenta, buffered aqueous solution 10 μg

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • C50416-Carboxyfluorescein diacetate 10 mgFDS

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Kasturi Muthoosamy et al.
Scientific reports, 6, 32808-32808 (2016-09-07)
Metastasis of lung carcinoma to breast and vice versa accounts for one of the vast majority of cancer deaths. Synergistic treatments are proven to be the effective method to inhibit malignant cell proliferation. It is highly advantageous to use the
Rajeev Kumar Pandey et al.
PloS one, 6(2), e17093-e17093 (2011-02-25)
Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that
Christo Paul Preethy et al.
Journal of pharmacology & pharmacotherapeutics, 3(1), 26-34 (2012-03-01)
To find the efficacy of serial extracts of Anisomeles malabarica in inhibiting proliferation of and inducing apoptosis in human cervical cancer cells, SiHa and ME 180, that are HPV 16-positive. The whole plant was extracted in n-hexane, chloroform, ethyl acetate
Mohamed Asik R et al.
Materials science & engineering. C, Materials for biological applications, 103, 109840-109840 (2019-07-28)
Development of novel approach for cancer therapy, sparing healthy normal cells overcoming the limitation of available therapies is of prime importance for cervical cancer treatment. Recently metal oxide based chemotherapeutics has emanated as a promising approach for cancer therapy. Hence
Andre F Steinert et al.
Arthritis research & therapy, 11(5), R148-R148 (2009-10-06)
The present study compares bone morphogenetic protein (BMP)-4 and BMP-2 gene transfer as agents of chondrogenesis and hypertrophy in human primary mesenchymal stem cells (MSCs) maintained as pellet cultures. Adenoviral vectors carrying cDNA encoding human BMP-4 (Ad.BMP-4) were constructed by
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Articles

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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