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A8228

Sigma-Aldrich

Anti-hABH2 antibody, Mouse monoclonal

clone hABH2-7, purified from hybridoma cell culture

Synonyme(s) :

Anti-Human AlkB Homologue 2, Monoclonal Anti-hABH2 antibody produced in mouse

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About This Item

Numéro MDL:
MFCD06412251
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

hABH2-7, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~30 kDa

Espèces réactives

human

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of 293T cells

Isotype

IgG1

Numéro d'accès UniProt

Q6NS38

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ALKBH2(121642)

Catégories apparentées

Description générale

Anti-hABH2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH2-7 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human hABH2, conjugated to KLH. Human alkB homolog 1, histone H2A dioxygenase (hABH2) shares homology with the AlkB protein in bacteria. hABH2 localizes to the nucleoplasm and occasionally to the nucleoli.

Application

Mouse Monoclonal Anti-hABH2 antibody has been used for western blot assays. The antibody can also be used for IHC, ELISA, microarray, and immunoprecipitation studies.

Actions biochimiques/physiologiques

Removal of the methyl groups of 1-methyladenine (1-meA) and 3-methylcystosine (3-meC) to their unmodified forms is done by human alkB homolog 1, histone H2A dioxygenase (hABH2) and hABH3. hABH2 acts on double stranded DNA.
hABH2 is a Fe (II) dependent dioxygenase involved in the repair of alkylation-induced damage in double stranded DNA. Alterations in hABH2 have been linked to pediatric brain tumor and gastric cancer.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Minimal methylated substrate and extended substrate range of Escherichia coli AlkB protein, a 1-methyladenine-DNA dioxygenase
Koivisto P, et al.
The Journal of Biological Chemistry, 278(45), 44348-44354 (2003)
Reversal of DNA alkylation damage by two human dioxygenases
Duncan T, et al.
Proceedings of the National Academy of Sciences of the USA, 99(26), 16660-16665 (2002)
Wei Gao et al.
Journal of gastroenterology and hepatology, 26(3), 577-584 (2010-12-16)
Methyl or 1, N(6) -ethenoadenine base lesions are frequent and highly-mutagenic or -carcinogenic events in mammalian DNA. Human AlkB homologue-2 (hABH2), a homologue of the Escherichia coli AlkB protein, has been found to be the principal dioxygenase for the repair
Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA
Aas PA, et al.
Nature, 421(6925), 859-859 (2003)
Valentina Cetica et al.
Journal of neuro-oncology, 94(2), 195-201 (2009-03-18)
Alkylating agents, commonly used for brain tumor therapy, induce DNA and RNA lesions that, if not repaired, drive cells to apoptosis. Thus, cellular mechanisms that are responsible for nucleic acid repair are possibly involved in drug resistance. This work analyzes
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