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A6561

Sigma-Aldrich

Monoclonal Anti-Biotin–Alkaline Phosphatase antibody produced in mouse

clone BN-34, purified immunoglobulin, buffered aqueous glycerol solution

Synonyme(s) :

Monoclonal Anti-Biotin

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

mouse

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

BN-34, monoclonal

Forme

buffered aqueous glycerol solution

Technique(s)

dot blot: 1:10,000 using human IgG and biotin-monoclonal anti-human IgG
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20 using human tonsil and biotin-monoclonal anti-human IgG
indirect ELISA: 1:30,000 using human IgG and biotin-monoclonal anti-human IgG
western blot (chemiluminescent): 1:10,000 using human IgG and biotin-monoclonal anti-human IgG

Isotype

IgG1

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Monoclonal Anti-Biotin (mouse IgG1 isotype) is derived from the BN-34 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with biotin-keyhole limpet hemocyanin (KLH).
The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Immunogène

Biotin-KLH conjugate

Application

Monoclonal Anti-Biotin-Alkaline Phosphatase antibody produced in mouse has been used in:
  • blotting (western), Southern and dot blots)
  • immunohistology
  • in situ nucleic acids hybridization
  • enzyme linked immunosorbent assay (ELISA)
  • fluorescent activated cell-sorting (FACS)
  • electron microscopy

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Actions biochimiques/physiologiques

Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
The high binding affinity of biotin to egg white or bacteria-derived avidin has been exploited in the design of immunoassays and immunohistologic staining techniques. Conventional immunoassay methods are improved using monoclonal anti-biotin, which enhances the sensitivity of avidin-biotin immunoassays by adding a second layer of enzyme via the biotin specific monoclonal antibody.

Forme physique

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Angewandte Chemie (International ed. in English), 43(7), 900-903 (2004-02-10)
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Biomacromolecules, 9(7), 2056-2062 (2008-06-26)
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Rocio Rodriguez-Melendez et al.
The Journal of nutritional biochemistry, 14(12), 680-690 (2003-12-24)
In mammals, biotin serves as coenzyme for four carboxylases, which play essential roles in the metabolism of glucose, amino acids, and fatty acids. Biotin deficiency causes decreased rates of cell proliferation, impaired immune function, and abnormal fetal development. Evidence is
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