A5168

Adénosine déaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

• Synonyme(s) : Adénosine aminohydrolase
• Numéro CAS: 9026-93-1
• Numéro de classification (Commission des enzymes): 3.5.4.4 (BRENDA, IUBMB)
• Numéro CE : 232-817-5
• Numéro MDL: MFCD00081287
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: bovine spleen
• Niveau de qualité: 200
• Type: Type IX
• Forme: ammonium sulfate suspension
• Activité spécifique: 150-200 units/mg protein
• Poids mol.: 32.5-33 kDa
• Numéro d'accès UniProt: P56658
• Activité étrangère: 5′-AMP deaminase ≤0.01%; alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• Température de stockage: 2-8°C
• Informations sur le gène: cow ... ADA(280712)

Description

Description générale

Poids moléculaire 32,5-33 kDa

pI : 4,85

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.

Application

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Actions biochimiques/physiologiques

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

Définition de l'unité

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Forme physique

Suspension in 3.2 M (NH₄)₂SO₄, 0.01 M potassium phosphate, pH 6.0

Remarque sur l'analyse

Protein determined by biuret.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)