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A3415

Sigma-Aldrich

Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey

affinity isolated antibody, buffered aqueous solution

Synonyme(s) :

Anti Sheep Antibody, Anti Sheep Antibody - Anti-Sheep IgG (whole molecule)–Peroxidase antibody produced in donkey, Anti Sheep Hrp

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About This Item

Numéro MDL:
MFCD00162847
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

donkey

Conjugué

peroxidase conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Espèces réactives

sheep

Technique(s)

direct ELISA: 1:10,000

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

IgGs are glycoprotein antibodies that modulate several immune responses. Sheep IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-sheep IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Anti-Sheep IgG (whole molecule)-Peroxidase antibody reacts with all sheep immunoglobulins.

Immunogène

Purified sheep IgG

Application

Anti-Sheep IgG (whole molecule)-Peroxidase antibody is suitable for use in immunoblot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
rElisas and CHEkiT Elisas were performed on serum from Sarda milking ewes using HRP-conjugated donkey anti-sheep IgG at1:1000. The antibody was incubated for 30 minutes at 37 degrees.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative

Notes préparatoires

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Mentions de danger

H315,H317,H319,H411

Classification des risques

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

María J Torres et al.
Frontiers in microbiology, 8, 1621-1621 (2017-09-16)
The powerful greenhouse gas, nitrous oxide (N
Max Werth et al.
Hepatology (Baltimore, Md.), 44(4), 967-975 (2006-09-29)
In mammalian liver, high glutamine synthetase (GS) expression is restricted to hepatocytes surrounding the terminal venules. The most important enhancer of the GS gene is located approximately 2520 base pairs (bp) upstream from the transcriptional start point. The nature of
ACE inhibition increases expression of the ETB receptor in kidneys of mice with unilateral obstruction.
Moridaira, K., et al.
American Journal of Physiology: Renal Physiology, 284(1), F209-F217 (2003)
Increased contractility of diabetic rabbit corpora smooth muscle in response to endothelin is mediated via Rho-kinase β
Chang, S., et al.
The Journal of Urology, 15, 53-62 (2003)
Marcus G Davey et al.
PloS one, 12(1), e0171132-e0171132 (2017-02-01)
A major limitation to adeno-associated virus (AAV) gene therapy is the generation of host immune responses to viral vector antigens and the transgene product. The ability to induce immune tolerance to foreign protein has the potential to overcome this host
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