93773

TWEEN^® 20

viscosity 250-450 mPa.s (25 °C)

• Synonyme(s) : Monolaurate de polyoxyéthylènesorbitan, Monolaurate de polyéthylèneglycolsorbitan
• Numéro CAS: 9005-64-5
• Numéro MDL: MFCD00165986
• Code UNSPSC : 12161900
• Nomenclature NACRES : NA.21

Propriétés

• Description: non-ionic
• Forme: viscous liquid
• Poids mol.: ~1228
• Technique(s): DNA extraction: suitable; RNA extraction: suitable; cell culture | mammalian: suitable; protein purification: suitable
• Indice de réfraction: n20/D 1.468 (lit.); n20/D 1.471
• Viscosité: 250-450 mPa.s(25 °C)
• HLB: 16.7±1.0
• Densité: 1.11 g/mL at 20 °C; 1.095 g/mL at 25 °C (lit.)
• Chaîne SMILES: CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
• InChI: 1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
• Clé InChI: HMFKFHLTUCJZJO-UHFFFAOYSA-N

Description

Description générale

Tween^®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

Tween^® 20 is widely used in several biochemical applications. It has been used:

• as an emulsifying agent for the preparation of stable oil-in-water emulsions
• in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
• for lysing mammalian cells at a concentration of 0.005 to 0.5%
• for rehydrating tissue before in situ hybridization

Détergent non ionique

Actions biochimiques/physiologiques

Tween^® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.

Caractéristiques et avantages

• Suitable for protein purification and DNA and RNA extraction
• Suitable for mammalian cell culture
• High purity product for research applications

Attention

may get turbid on storage, heat to clear

Autres remarques

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Informations légales

TWEEN is a registered trademark of Croda International PLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): 527.0 °F - Pensky-Martens closed cup
• Point d'éclair (°C): 275 °C - Pensky-Martens closed cup
• Équipement de protection individuelle: Eyeshields, Gloves