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93658

Sigma-Aldrich

Esterase Pyrobaculum calidifontis, recombinant

recombinant, expressed in E. coli, ≥2.0 U/mg

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About This Item

Numéro CAS:
Numéro de classification (Commission des enzymes):
Numéro CE :
Code UNSPSC :
12352204

Produit recombinant

expressed in E. coli

Forme

crystalline
crystals
powder or flakes

Activité spécifique

≥2.0 U/mg

Température de stockage

−20°C

Application

Esterase, from Pyrobaculum calidifontis, may be used in structural biology research.

Actions biochimiques/physiologiques

An esterase is a hydrolase that splits esters into acids and alcohols.

Conditionnement

Bottomless glass bottle. Contents are inside inserted fused cone.

Définition de l'unité

1 U corresponds to the amount of enzyme which converts 1 μmol 4-nitrophenyl-L-acetate per minute at pH 7.5 and 30°C.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Gottfried J Palm et al.
Applied microbiology and biotechnology, 91(4), 1061-1072 (2011-05-27)
The highly thermostable esterase from the hyperthermophilic archaeon Pyrobaculum calidifontis VA1 (PestE) shows high enantioselectivity (E > 100) in the kinetic resolution of racemic chiral carboxylic acids, but little selectivity towards acetates of tertiary alcohols (E = 2-4). To explain
XieMei Tang et al.
Critical reviews in eukaryotic gene expression, 22(3), 179-187 (2012-11-13)
Tuberculosis remains one of the most prevalent and deadly infectious diseases, largely due to the emergence of multidrug-resistant and extensive drug-resistant Mycobacterium tuberculosis, especially the coinfection with HIV. Mycobacterium Ag85 complex (Ag85A, B, and C), with a carboxylesterase consensus sequence
Zhe-Yi Hu et al.
Analytical and bioanalytical chemistry, 405(5), 1695-1704 (2012-12-15)
Dabigatran etexilate (DABE) is an oral prodrug that is rapidly converted by esterases to dabigatran (DAB), a direct inhibitor of thrombin. To elucidate the esterase-mediated metabolic pathway of DABE, a high-performance liquid chromatography/mass spectrometry based metabolite identification and semi-quantitative estimation
Keun Na et al.
International journal of cancer, 133(2), 408-415 (2013-01-16)
Although alpha-fetoprotein (AFP) is currently the major serologic biomarker for hepatocellular carcinoma (HCC), it cannot efficiently distinguish this cancer from other forms of liver disease in early diagnosis due to its low sensitivity. The aim of this study is to
Takashi Nishiyama et al.
Acta crystallographica. Section D, Biological crystallography, 69(Pt 1), 44-51 (2013-01-01)
Bacterial biofilm formation is an extremely widespread phenomenon involving the secretion of a protective exopolysaccharide matrix which helps the bacteria to attach to surfaces and to overcome a variety of stresses in different environments. This matrix may also include proteins

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