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83330

Sigma-Aldrich

Pyruvate Kinase from rabbit muscle

crystalline suspension in 3.8 M ammonium sulfate solution, ~350 U/mg protein (10-40 mg/ml)

Synonyme(s) :

ATP:pyruvate 2-O-phosphotransferase, PK

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About This Item

Numéro CAS:
9001-59-6
Numéro de classification (Commission des enzymes):
2.7.1.40 (BRENDA, IUBMB)
Numéro CE :
232-616-2
Numéro MDL:
MFCD00081946
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54

Forme

suspension

Qualité

crystalline suspension in 3.8 M ammonium sulfate solution

Activité spécifique

~350 U/mg protein (10-40 mg/ml)

Poids mol.

Mr ~230000

pH

~6

Activité étrangère

lactate dehydrogenase ≤0.01%

Température de stockage

2-8°C

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Actions biochimiques/physiologiques

Molecular Weight: 237 kDa and exists as a tetramer of four equal subunits of molecular weight 57 kDa.
Isoelectric Point: 7.6
Optimal pH: ∼7.5
Optimal Temperature: 25°C
ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path
Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Pyruvate kinase catalyzes the conversion of phosphoenolpyruvate to pyruvate, whitch is the rate-limiting final step in glycolysis.

Définition de l'unité

1 U corresponds to the amount of enzyme which catalyzes the transphosphorylation of 1 μmol PEP per min at pH 7.4 and 25 °C

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Jidong Cheng et al.
Genes to cells : devoted to molecular & cellular mechanisms, 17(11), 913-922 (2012-10-20)
AMP deaminase (AMPD) catalyzes AMP to IMP and plays an important role in energy charge and nucleotide metabolism. Human AMPD3 deficiency is a type of erythrocyte-specific enzyme deficiency found in individuals without clinical symptoms, although an increased level of ATP
Patrícia Machado et al.
PloS one, 7(10), e47071-e47071 (2012-10-20)
Pyruvate kinase (PK) deficiency, causing hemolytic anemia, has been associated to malaria protection and its prevalence in sub-Saharan Africa is not known so far. This work shows the results of a study undertaken to determine PK deficiency occurrence in some
Karl Kochanowski et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(3), 1130-1135 (2013-01-02)
Regulation of metabolic operation in response to extracellular cues is crucial for cells' survival. Next to the canonical nutrient sensors, which measure the concentration of nutrients, recently intracellular "metabolic flux" was proposed as a novel impetus for metabolic regulation. According
Beverly A Teicher et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 18(20), 5537-5545 (2012-10-17)
The understanding that oncogenes can have profound effects on cellular metabolism and the discovery of mutations and alterations in several metabolism-related enzymes--isocitrate dehydrogenase 1 (IDH1), isocitrate dehydrogenase 2 (IDH2), succinate dehydrogenase (SDH), fumarate hydratase (FH), and pyruvate kinase M2 (PKM2)--has
James M Urness et al.
Biochemistry, 52(1), 1-3 (2012-12-22)
A series of substrate analogues has been used to determine which chemical moieties of the substrate phosphoenolpyruvate (PEP) contribute to the allosteric inhibition of rabbit muscle pyruvate kinase by phenylalanine. Replacing the carboxyl group of the substrate with a methyl
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