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Sigma-Aldrich

Chlorure de sodium solution

BioUltra, for molecular biology, ~5 M in H2O

Synonyme(s) :

Halite, NaCl, sodium chloride

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About This Item

Formule linéaire :
NaCl
Numéro CAS:
7647-14-5
Poids moléculaire :
58.44
Numéro Beilstein :
3534976
Numéro MDL:
MFCD00003477
Code UNSPSC :
12352302
ID de substance PubChem :
57652296
Nomenclature NACRES :
NA.25

Qualité

for molecular biology

Gamme de produits

BioUltra

Forme

liquid

Concentration

~5 M in H2O

Technique(s)

DNA extraction: suitable
LAMP: suitable

Impuretés

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

Indice de réfraction

n20/D 1.378

pH

5.0-7.0 (25 °C)

Solubilité

water: soluble

Densité

1.186 g/mL (5 M in water)

Traces de cations

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

Absorption UV

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

Adéquation

suitable for molecular biology

Application(s)

genomic analysis

Chaîne SMILES 

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

Clé InChI

FAPWRFPIFSIZLT-UHFFFAOYSA-M

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Description générale

Sodium chloride is a vital nutrient that aids normal body functions in humans and animals. This chemical is commonly found in nature with a wide variety of biochemical applications, like intravenous fluids and density gradients. It is also used as a diluent to increase ionic strength in buffers or culture media. Sodium chloride is crucial for maintaining plasma osmolality and the extracellular fluid volume of the body. Excess NaCl leads to increased blood pressure, which has implications for cardiovascular and renal diseases. Sterile NaCl is a component of eye drop solutions. Traditionally, it has been used in high concentrations to preserve food since most bacteria cannot grow in high-salt conditions.

Application

Sodium chloride solution has been used as a component of:
  • polyethylene glycol (PEG) buffer for the purification of double-tagged products
  • NT2 buffer for the preparation of antibody-coated Protein A Sepharose (PAS) beads and immunoprecipitation of ribonucleoproteins (RNPs)
  • chilled lysis buffer for the isolation of single nuclei from mice kidney

Caractéristiques et avantages

  • Geologically stable
  • Suitable for purification, precipitation, and crystallization

Autres remarques

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

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Journal of the American College of Cardiology, 66(8), 892-900 (2015-08-22)
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Journal of clinical microbiology, 52(7), 2359-2364 (2014-04-25)
Multidrug-resistant Acinetobacter baumannii isolates, particularly those that produce carbapenemases, are increasingly reported worldwide. The biochemically based Carba NP test, extensively validated for the detection of carbapenemase producers among Enterobacteriaceae and Pseudomonas spp., has been modified to detect carbapenemase production in
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