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57180

Sigma-Aldrich

Indo 1

for fluorescence, ≥80.0% (HPLC)

Synonyme(s) :

4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt

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About This Item

Formule empirique (notation de Hill):
C32H26K5N3O12
Numéro CAS:
Poids moléculaire :
840.05
Numéro MDL:
Code UNSPSC :
12352200
ID de substance PubChem :
Nomenclature NACRES :
NA.32

Qualité

for fluorescence

Pureté

≥80.0% (HPLC)

Forme

solid

Fluorescence

λex 331 nm; λem 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca2+
λex 349 nm; λem 482 nm (100mM KCI)

Adéquation

in accordance for fluorescence

Température de stockage

−20°C

Chaîne SMILES 

[K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1

InChI

1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5

Clé InChI

VGZPRMSXVNDMAN-UHFFFAOYSA-I

Application

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Autres remarques

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.

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