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3447-020-01

Sigma-Aldrich

Cultrex 3-D Culture Matrix™ Rat Collagen I

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About This Item

Code UNSPSC :
12352200

Source biologique

rat

Stérilité

sterile

Poids mol.

300 kDa

Concentration

5 mg/mL

Technique(s)

cell culture | mammalian: suitable

Solubilité

water: miscible

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

The 3-D Culture Matrix™ Rat Collagen I may be used as a gel on which to grow cells or a medium additive, alone or in concert with other basement membrane components, to study cellular growth and differentiation in three dimensions in vitro. Type I Collagen is the major structural component of extracellular matrices found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300 kDa molecule composed of two alpha1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. The 5 mg/ml concentration is very viscous for researchers wanting a thicker Collagen.

Forme physique

Solution in 20 mM Acetic Acid, pH 3.4 - 3.6

Autres remarques

Functional Assay: 3-D Culture: Collagen I forms a gel when diluted to 0.4 mg/ml at neutral pH and promotes attachment and growth of murine endothelial SVEC4-10 cells. Cell Attachment: Tested for the ability to promote cell attachment and spreading of HT1080 human fibrosarcoma cells.
Sterility Testing: No bacterial or fungal growth detected after incubation at 37°C for 14 days following USP XXIV Chapter 71 sterility test. No mycoplasma contamination detected by PCR.Endotoxin concentrations = 20 EU/ml by LAL assay.
Storage Conditions and product stability: Product is stable for a minimum of 3 months from date of shipment if stored at 4°C. Do Not Freeze.

Informations légales

3-D Culture Matrix is a trademark of Trevigen, Inc.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Claudia Beaurivage et al.
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Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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