17490

Bromocresol Purple

for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

• Synonyme(s) : 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form
• Formule empirique (notation de Hill): C₂₁H₁₆Br₂O₅S
• Numéro CAS: 115-40-2
• Poids moléculaire : 540.22
• Numéro Beilstein : 359618
• Numéro CE : 204-087-8
• Numéro MDL: MFCD00011681
• Code UNSPSC : 12171500
• ID de substance PubChem : 57647609
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: for microscopy (Hist., Vit.); indicator (pH 5.2-6.8)
• Forme: powder
• Technique(s): titration: suitable
• pH: 5.2-6.8, yellow to purple
• Pf: 240 °C (dec.) (lit.)
• Solubilité: hot ethanol: 0.1 g/10 mL
• εmax: ≥400 at 414-424 nm in methanol
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
• InChI: 1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
• Clé InChI: ABIUHPWEYMSGSR-UHFFFAOYSA-N

Description

Description générale

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.

Application

Spectrophotometric determination of serum-albumin

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves