T17917

Tricine

≥99% (titration), reagent grade, crystalline

• Synonyme(s) : N-[Tris(hydroxyméthyl)méthyl]glycine
• Formule linéaire : (HOCH₂)₃CNHCH₂CO₂H
• Numéro CAS: 5704-04-1
• Poids moléculaire : 179.17
• Numéro Beilstein : 1937804
• Numéro MDL: MFCD00004277
• Code UNSPSC : 12161700
• Nomenclature NACRES : NA.25

Propriétés

• Source biologique: synthetic
• Niveau de qualité: 200
• Qualité: reagent grade
• Pureté: ≥99% (titration)
• Forme: crystalline
• Conditions de stockage: (Tightly closed. Dry)
• Impuretés: <0.5% water
• Couleur: white
• Plage de pH utile: 7.4-8.8
• pKa (25 °C): 8.1
• Pf: 187 °C
• Solubilité: water: 0.1 M, clear, colorless
• Traces de cations: Pb: ≤5 ppm
• Température de stockage: room temp
• Chaîne SMILES: OCC(CO)(CO)NCC(O)=O
• InChI: 1S/C6H13NO5/c8-2-6(3-9,4-10)7-1-5(11)12/h7-10H,1-4H2,(H,11,12)
• Clé InChI: SEQKRHFRPICQDD-UHFFFAOYSA-N

Description

Description générale

Tricine, a zwitterionic biological buffer, is commonly employed in research applications such as enzyme assays, electrophoresis, cell biology, and biochemical studies. Its increased negative charge compared to glycine ensures rapid migration during electrophoresis, facilitating efficient separation of low-molecular-weight proteins in gels with lower acrylamide percentages. Moreover, Tricine may also be used in cryopreservation media, aiding in the preservation of tissues and organs.

Tricine′s valuable properties for biological research include its zwitterionic nature, wide buffering range (pH 7.4-8.8), classification as a Good′s buffering agent, non-toxicity to cells, and high water solubility and clarity. These attributes make it suitable for metal-sensitive systems, stable pH maintenance, optimal biological applications, cell biology studies, and accurate measurements. Additionally, Tricine may find utilization in capillary zone electrophoresis, high-performance liquid chromatography, and ion exchange chromatography, demonstrating adaptability across analytical techniques.

Application

Tricine is intended to use for:

• Electrophoresis To separate low-weight peptides and proteins
• Crystallization of proteins of varying pH
• Resuspension of cell pellets
• Enzyme assays
• Tissue culture

Constituant de tampons destinés à la séparation de peptides de faible poids moléculaire.

Caractéristiques et avantages

• Ideal as a Biological buffer for Cell Biology and Biochemical research
• Effective Buffering from pH 7.4 - 8.8 with a pKa of 8.1 (25 °C)

Autres remarques

For additional information on our range of Biochemicals, please complete this form.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable