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51490

Millipore

Hektoen Enteric Agar

suitable for microbiology, NutriSelect® Plus

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About This Item

Code UNSPSC :
41171606
Nomenclature NACRES :
NA.74

Stérilité

non-sterile

Niveau de qualité

200

Forme

powder

Durée de conservation

limited shelf life, expiry date on the label

Composition

acid fuchsin, 0.1 g/L
agar, 15.0 g/L
ammonium ferric citrate, 1.5 g/L
bile salts, 9 g/L
bromothymol blue, 0.065 g/L
lactose, 12 g/L
mixed peptone, 12 g/L
salicin, 2 g/L
sodium chloride, 5 g/L
sodium thiosulfate, 5 g/L
sucrose, 12 g/L
yeast extract, 3 g/L

Fabricant/nom de marque

NutriSelect® Plus

Technique(s)

microbiological culture: suitable

pH final

7.5±0.2 (25 °C)

Application(s)

clinical testing
environmental
food and beverages
water monitoring
microbiology

Adéquation

selective and differential for Salmonella spp.
selective and differential for Shigella spp.
selective and differential for bacteria (General Media)

Application

Hektoen enteric agar is a type of primary standard media, which may be commonly used in the selective detection and isolation of Salmonella in food microbiology labs and in human stool specimens.

Notes préparatoires

Suspend 76.67 g in 1 litre distilled water and let it soak. Heat up by constant stirring until boiling. Cool to 55 - 60 °C and pour into sterile plates. Do not autoclave. The Medium is very thermolabile and thus overheating should be avoided.

Autres remarques

Plating medium for the isolation of enteric pathogens; Isolation of Shigellae

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Classification des risques

Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificats d'analyse (COA)

Lot/Batch Number
BCCL8834
BCCG1674
BCCF4105
BCCD6192
BCCD4843

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Entis P
Food Microbiology: The Laboratory (2002)
Comparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools
Perez.MJ, et al.
Journal of Clinical Microbiology, 41, 1130-1134 (2003)
S. King et al.
Applied Microbiology, 16, 557-557 (1968)
W I Taylor et al.
Applied microbiology, 21(1), 32-37 (1971-01-01)
Two enrichment broths and four plating media were compared for efficiency of detection of enteric pathogens from 1,597 stool specimens. Of 170 salmonellae isolated from the composite of all methods, direct streaking yielded but 54%, whereas enrichment in gram-negative broth
S King et al.
Applied microbiology, 16(4), 579-581 (1968-04-01)
During this study, 2,855 stool specimens from patients at Cook County Hospital were cultured for enteric pathogens. Hektoen Enteric Agar (HE) was compared with E M B and S S Agars by replicate samplings with both direct and indirect methods.

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