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SFBRKB

Roche

KAPA SYBR® FAST

suitable for qPCR, 2 ×, Bio-Rad iCycler®

Synonyme(s) :

qPCR

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About This Item

Code UNSPSC :
41106300
Nomenclature NACRES :
NA.55

Niveau de qualité

100

Utilisation

sufficient for 100 reactions
 sufficient for 1000 reactions
 sufficient for 500 reactions

Durée de conservation

≤12 mo.

Caractéristiques

dNTPs included
hotstart

Conditionnement

kit of 1 mL (100 x 20 μL rxn; KK4606)
kit of 10 mL (1000 x 20 μL rxn; KK4608)
kit of 5 mL (500 x 20 μL rxn; KK4607)

Fabricant/nom de marque

Roche

Concentration

2 ×

Technique(s)

qPCR: suitable

Entrée

purified DNA

Compatibilité

for use with Bio-Rad iCycler

Méthode de détection

SYBR® Green

Température de stockage

−20°C

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Description générale

KAPA SYBR FAST qPCR Kits for Bio-Rad iCycler® contain the first DNA polymerase engineered via directed evolution to be more tolerant of SYBR Green I dye inhibition.

The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.

Application

KAPA2G Fast HotStart® ReadyMix™ has been used for:
  • Gene expression
  • Gene knockdown validation
  • Microarray validation
  • ChIP analysis
  • Low copy detection
  • Absolute quantification of NGS libraries
  • Real-time quantitative reverse transcription-PCR (qRT-PCR)
  • quantitative PCR (qPCR)

Caractéristiques et avantages

Quantitate changes in gene expression more accurately
  • High reaction efficiency between 95 - 105% improves accuracy and reproducibility.
  • Unbiased efficiency across a wide range of GC contents and amplicon lengths.

Detect low copy and difficult targets consistently
  • Improved processivity results in earlier Cq scores.
  • Higher fluorescence detection across varying AT- and GC-rich targets.
  • Novel enzyme is resistant to SYBR®inhibition.

Complete real-time PCR runs in just 40 minutes
  • 55% shorter run times with fast cycling protocol.
  • Maintain high performance when switching from standard to fast protocols.

Quick Notes:
  • This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
  • The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
  • 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
  • For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
  • Do not exceed 25 μL reaction volumes.

Conditionnement

Ships on Dry Ice in some cases however, shipping conditions may vary in certain regions

Qualité

All kit components are subjected to stringent functional quality control, are free of detectable contaminating exo and endonuclease activity, and meet strict requirements with respect to DNA contamination.

Notes préparatoires

Always ensure that components have been fully thawed and thoroughly mixed before use. KAPA SYBR® FAST qPCR Master Mix (2X) may not freeze solidly, even when stored at -20°C.

The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) for Bio-Rad® iCycler® and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.

KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.

Autres remarques

For Research Use Only. Not for use in diagnostic procedures.

Informations légales

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
HOTSTART is a registered trademark of Molecular BioProducts, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
iCycler is a registered trademark of Bio-Rad

Composants de kit seuls

Réf. du produit
Description
  • KAPA SYBR® FAST DNA Polymerase
  • reaction buffer
  • dNTPs
  • SYBR® Green I dye
  • MgCl2 2.5 mM

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H371

Classification des risques

STOT SE 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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