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RTRYP-RO

Roche

Trypsin recombinant, Proteomics Grade

recombinant enzyme expressed in Pichia pastoris

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About This Item

Numéro de classification (Commission des enzymes):
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.21

Produit recombinant

expressed in Pichia pastoris

Niveau de qualité

Pureté

95% (gel filtration)

Forme

lyophilized

Activité spécifique

≥150 units/mg protein

Conditionnement

pkg of 4 × 100 μg (03708969001)
pkg of 4 × 25 μg (03708985001)

Fabricant/nom de marque

Roche

Température de stockage

−20°C

Description générale

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Spécificité

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Application

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

Notes préparatoires

Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Reconstitution

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Pictogrammes

Exclamation markHealth hazard

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


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Consulter la Bibliothèque de documents

Alexander Schmidt et al.
Methods in molecular biology (Clifton, N.J.), 492, 21-38 (2009-02-26)
Proteomics may be defined as the systematic analysis of proteins expressed in a given organism (Electrophoresis 16:1090-1094, 1995). Important technical innovations in mass spectrometry (MS), protein identification methods, and database annotation, over the past decade, now make it possible to
A Acera et al.
Eye (London, England), 25(9), 1225-1233 (2011-06-28)
To analyze tear protein profile variations in patients with keratoconus (KC) and to compare them with those of control subjects. Tears from 12 normal subjects and 12 patients with KC were analyzed by two-dimensional gel electrophoresis (2-DE) and liquid chromatography-mass

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