11772457001
Roche
TUNEL AP
sufficient for 70 tests, solution, pkg of 3.5 mL
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About This Item
Code UNSPSC :
41105600
Produits recommandés
Forme
solution
Utilisation
sufficient for 70 tests
Conditionnement
pkg of 3.5 mL
Fabricant/nom de marque
Roche
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
TUNEL AP is an alkaline phosphatase-labeled antibody used for the in situ detection of apoptosis (programmed cell death) with the TUNEL reaction followed by transmission light microscopy.
The tailing reaction using TdT, also named ISEL (in situ end labeling) or TUNEL (TdT-mediated dUTP nick end labeling), has several advantages in comparison to the in situ nick translation (ISNT) using DNA polymerase:
- Label intensity of apoptotic cells is higher with TUNEL compared to ISNT, resulting in an increased sensitivity.
- Kinetics of nucleotide incorporation is very rapid with TUNEL compared to the ISNT.
- TUNEL preferentially labels apoptotic cells compared to necrotic cells.
Application
TUNEL AP is an antibody that is used to convert fluorescence-based TUNEL assays into colorimetric assays suited for light microscopy. The conversion is performed by binding of an anti-fluorescein antibody to FITC-dUTP. The antibody is labeled with alkaline phosphatase (AP). The AP is visualized with a precipitating substrate, such as Fast Red or NBT/BCIP.
Composants
Anti-fluorescein antibody, Fab fragment from sheep, conjugated with alkaline phosphatase (AP). Ready-to-use solution.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Skin Sens. 1
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
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