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11418165001

Roche

Biotin Protein Labeling Kit

sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA

Synonyme(s) :

Biotin, protein labeling

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About This Item

Code UNSPSC :
12352200

Utilisation

sufficient for 5 labeling reactions

Niveau de qualité

100

Conditionnement

kit of 1 (5 components)

Fabricant/nom de marque

Roche

Technique(s)

ELISA: suitable
immunoblotting: suitable

Température de stockage

2-8°C

Description générale

For labeling of proteins with biotin.
Free amino acids of the protein to be labeled react with D-biotinoyl-ε-aminocaproic acid-N-hydroxy-succinimide ester (biotin-7-NHS) by forming a stable amino bond. Non reacted biotin-7-NHS is separated on a Sephadex G-25 column.

Application

Biotin protein labeling kit has been used to produce biotinylated denatured bovine serum albumin (d-BSA) for coating cadmium telluride (CdTe) quantum dot (QD) conjugates.
The kit is suitable for the labeling of proteins with biotin for immunochemistry, immunoblotting, and ELISA.

Conditionnement

1 kit containing 5 components.

Qualité

Function tested.

Notes préparatoires

Working solution: Preparation of the solutions
  • Blocking solution: Dissolve the contents of the bottle blocking reagent (green screw cap) in 300 ml double-distilled water.
  • PBS solution: Dissolve the contents of the bottle PBS (blue screw cap) in 1 I double-distilled water.
  • Biotin-7-NHS solution: The solution should be freshly prepared before labeling. Add to the contents of the reaction vial which includes biotin-7-NHS 250 μI DMSO and vortex the solution several times. The resulting concentration of the biotin-7-NHS solution is 20 mg/ml. For the labeling mix, the volume of the biotin-7-NHS solution should not exceed 5% of the total reaction volume (e.g., add maximal 50 μl biotin-7-NHS solution to 1 ml protein solution).

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description
  • Blocking Reagent (green screw cap)
  • Phosphate Buffered Saline (PBS) (blue screw cap)
  • D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)
  • Dimethylsulfoxide (DMSO) (red screw cap)
  • Sephadex G-25 Columns

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

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