OP29
Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240)
liquid, clone PAb240, Calbiochem®
Synonyme(s) :
Mutant p53 Antibody
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
PAb240, monoclonal
Forme
liquid
Contient
≤0.1% sodium azide as preservative
Espèces réactives
mouse, rat, human, chicken, hamster, bovine
Ne doit pas réagir avec
Xenopus
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
do not freeze
Isotype
IgG1
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... TP53(7157)
Description générale
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions. Recognizes both the mutant and the wild-type p53 protein under denaturing conditions.
Recognizes the ~53 kDa mutant p53 protein under non-denaturing conditions by immunoprecipitation, immunofluorescence, and flow cytometry. Recognizes both mutant and wild-type p53 by immunoblotting and paraffin sections under denaturing conditions.
This Anti-p53 (Ab-3) (Mutant) Mouse mAb (PAb240) is validated for use in FC, Frozen Sections, Gel Shift, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-3) (Mutant).
Immunogène
Epitope: within amino acids 213-217
Human
a recombinant protein consisting of amino acids 14-389 of p53 fused to β-galactosidase
Application
Flow Cytometry (1-20 µg/ml)
Frozen Sections (10 µg/ml)
Gel Shift (see comments)
Immunoblotting (5 µg/ml)
Immunofluorescence (1-20 µg/ml, see application references)
Immunoprecipitation (1 µg per sample)
Paraffin Sections (see application references)
Frozen Sections (10 µg/ml)
Gel Shift (see comments)
Immunoblotting (5 µg/ml)
Immunofluorescence (1-20 µg/ml, see application references)
Immunoprecipitation (1 µg per sample)
Paraffin Sections (see application references)
Conditionnement
Please refer to vial label for lot-specific concentration.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Remarque sur l'analyse
Negative Control
SK-OV-3 cells
SK-OV-3 cells
Positive Control
A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
A431, Hs27 (wild-type p53), or SK-BR-3 cells or breast carcinoma tissue
Autres remarques
El-Deiry, W.S., et al. 1994. Cancer Res.54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.
Barak, Y., et al. 1993. EMBO J.12, 461.
Kastan, M.B., et al. 1992. Cell71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA89, 7491.
Lane, D.P. 1992. Nature358, 15.
Kastan, M.B., et al. 1991. Cancer Res.51, 6304.
Under non-denaturing conditions (immunoprecipitation, immunofluorescence and frozen sections), Anti-p53 (Ab-3) does not recognize normal (wild-type) p53 protein; it recognizes an epitope exposed by activating mutations or denaturation. In denaturing protocols (immunoblotting and paraffin sections), Anti-p53 (Ab-3) will recognize both mutant and wild-type p53. Will not recognize to some p53 molecules with mutations in the RHSVV epitope, but will react to TFIIIA, which has the RHSVV epitope. For gel shift assay, use Cat. No. OP29L and resuspend in 100 µl buffer. Antibody should be titrated for optimal results in individual systems.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Preethi H Gunaratne et al.
Cancer, 125(14), 2409-2422 (2019-04-24)
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