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MILLIPLEX^® Mouse High Sensitivity T Cell Panel- Premix Bulk (Space Saver) Packaging - Immunology Multiplex Assay

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

• Synonyme(s) : High Sensitivity T Cell Kit, Mouse T Cell Panel
• Code UNSPSC : 12161503
• eCl@ss : 32161000
• Nomenclature NACRES : NA.47

Propriétés

• Niveau de qualité: 200
• Espèces réactives: mouse
• Fabricant/nom de marque: Milliplex^®
• assay range: accuracy: 82-102%; sensitivity: 0.26-11.14 pg/mL; standard curve range: 0.10-400 pg/mL; (IL-4); standard curve range: 0.49-2,000 pg/mL; (IFNγ); standard curve range: 0.49-2,000 pg/mL; (IL-17A); standard curve range: 0.49-2,000 pg/mL; (IL-7); standard curve range: 0.49-2,000 pg/mL; (TNFα); standard curve range: 0.73-3,000 pg/mL; (KC/GROα/CXCL1); standard curve range: 0.98-4,000 pg/mL; (IL-2); standard curve range: 0.98-4,000 pg/mL; (IL-5); standard curve range: 0.98-4,000 pg/mL; (IL-6); standard curve range: 1.34-5,500 pg/mL; (IL-10); standard curve range: 2.93-12,000 pg/mL; (IL-12 (p70)); standard curve range: 3.17-13,000 pg/mL; (LIX); standard curve range: 3.42-14,000 pg/mL; (IL-13); standard curve range: 3.91-16,000 pg/mL; (IL-1α); standard curve range: 3.91-16,000 pg/mL; (IL-1β); standard curve range: 3.91-16,000 pg/mL; (MCP-1/CCL2); standard curve range: 6.1-25,000 pg/mL; (GM-CSF); standard curve range: 7.32-30,000 pg/mL; (MIP-2/CXCL2); inter-assay cv: <15%; intra-assay cv: <10%
• Technique(s): multiplexing: suitable
• Compatibilité: configured for Premixed
• Méthode de détection: fluorometric (Luminex xMAP)
• Conditions d'expédition: ambient

Description

Description générale

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.

Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders, and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.

The MILLIPLEX^® Mouse High Sensitivity T Cell Bead Panel is to be used for the simultaneous quantification of any or all of the following 18 mouse cytokines: GM-CSF, IFNγ, IL-1α, IL-1b, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC, LIX, MCP-1, MIP-2 and TNFα in mouse plasma, serum, and cell/tissue culture supernatant samples. This panel provides biomedical researchers quality tools for the study of low level inflammatory disease.

Panel Type: Cytokines/Chemokines

Application

• Analytes: GM-CSF, IFNγ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 (p70), IL-13, IL-17A, KC/CXCL1, LIX, MCP-1, MIP-2, TNF-α
• Recommended Sample type: serum, plasma, tissue culture or other media
• Recommended Sample dilution: neat or 1:2 diluted serum or plasma
• Assay Run Time: Overnight
• Research Category: Inflammation & Immunology
• Research Subcategory: Immune Response Metabolic Disorders Chronic Inflammatory Disease Cardiovascular Disease

Autres remarques

Please contact Technical Service for linearity of dilution.

Sensitivity: Please see protocol for sensitivity of individual analytes.

Informations légales

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Pictogrammes: GHS08,GHS05,GHS07,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H312 + H332,H317,H318,H373,H411
• Conseils de prudence: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Classification des risques: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Organes cibles: Respiratory Tract
• Code de la classe de stockage: 10 - Combustible liquids