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MABT523

Sigma-Aldrich

Anti-beta-Actin Antibody, clone RM112

clone RM112, from rabbit

Synonyme(s) :

Actin, cytoplasmic 1, Beta-actin, beta-Actin

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

RM112, monoclonal

Espèces réactives

human

Technique(s)

immunocytochemistry: suitable
western blot: suitable

Isotype

IgG

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... ACTB(60)

Description générale

Actin is a 43 kDa highly conserved structural protein. >> The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. Beta actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.

Immunogène

A peptide corresponding to the N-terminus of beta-Actin.

Application

Analyse par immunocytochimie (ICC) : A 1:200 dilution from a representative lot detected beta-Actin in A431 cells.

This Anti-beta-Actin Antibody, clone RM112 is validated for use in Western Blotting, Immunocytochemistry for the detection of beta-Actin.

Qualité

Produit évalué par western blotting sur du lysat de cellules A431.

Analyse par western blotting : A 1:1,000 dilution of this antibody detected beta-Actin in A431 cell lysate.

Description de la cible

Poids réel (observé) : env. 45 kDa. Des bandes non caractérisées peuvent être observées avec certains lysats.

Forme physique

Format : Produit purifié

Autres remarques

Concentration : Voir la fiche technique du lot concerné.

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Classe de danger pour l'eau (WGK)

WGK 2


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

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