MABS432

Anti-MEK1 Antibody, clone 6B7.1

clone 6B7.1, from mouse

• Synonyme(s) : MAP kinase kinase 1, MEK1, MKK1, MAPKK 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAPK/ERK kinase 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 6B7.1, monoclonal
• Espèces réactives: human
• Technique(s): western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_002746
• Numéro d'accès UniProt: Q02750
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MAP2K1(5604)

Description

Description générale

Dual specificity mitogen-activated protein kinase kinase 1 (EC 2.7.12.2; UniProt Q02750; also known as ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1) is encoded by the MAP2K1 (also known as CFC3, MAPKK1, MEK1, MKK1, PRKMK1) gene (Gene ID 5604) in human. MEK1 and the related MEK2 are dual specificity kinases of the MAP2K/STE7 kinase family. MEK1 and MEK2 share a high degree of sequence homology and perform similar functions. Upon activation by RAF, MEK1/2 catalyze the dual phosphorylation of threonine and tyrosine residues on ERK (MAPK) within a specific Thr–Glu–Tyr sequence at codons 202 to 204. This locks the kinase domain of MAPK into a catalytically competent conformation, ultimately leading to nuclear signals that regulate cellular growth and proliferation. MEK1/2 mutations have been reported to be associated with cardiofaciocutaneous syndrome 3 (CFC3) and cancers, including lung cancer, colorectal cancer, and melanomas.

Spécificité

Expected to react with both the MKK1a and MKK1b spliced isoforms.

Immunogène

Epitope: C-terminal region

GST-tagged recombinant protein corresponding to the C-terminal region of human MEK1.

Application

Anti-MEK1 Antibody, clone 6B7.1 is an antibody against MEK1 for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MEK1 in 10 µg of HeLa cell lysate.

Description de la cible

~45 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable