MABS2070

Anti-UGT1A10 Antibody, clone 20H1

clone 20H1, from mouse

• Synonyme(s) : UDP-glucuronosyltransferase 1-10, EC: 2.4.1.17, UGT1*10, UGT1-10, UGT1.10, UDP-glucuronosyltransferase 1-J, UGT-1J, UGT1J, UDP-glucuronosyltransferase 1A10
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 20H1, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μL
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG3κ
• Numéro d'accès NCBI: NP_061948.1
• Numéro d'accès UniProt: Q9HAW8
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... UGT1A10(54575)

Description

Description générale

UDP-glucuronosyltransferase 1-10 (UniProt: Q9HAW8; also known as EC: 2.4.1.17, UDPGT 1-10, UGT1*10, UGT1-10, UGT1.10, UDP-glucuronosyltransferase 1-J, UGT-1J, UGT1J, UDP-glucuronosyltransferase 1A10) is encoded by the UGT1A10 (also known as GNT1, UGT1) gene (Gene ID: 54575) in human. UDP-Glucuronosyltransferases (UGTs) are a major group of phase II drug metabolizing enzymes and each enzyme in this family displays a unique substrate specificity and tissue-specific expression patterns. UDP-Glucuronosyltransferases mediate the transfer of glucuronic acid from UDP-glucuronic acid to various aglycone substrates making them more hydrophilic and readily excretable. UGT1A10 is a single-pass membrane protein that is synthesized with a signal peptide (aa 1029), which is subsequently cleaved off in the mature form. Two isoforms of UGT1A10 have been described that are produced by alternative splicing. Both isoforms 1 and 2 are expressed in colon, esophagus, and small intestine. However, in liver only isoform 2 is expressed. Isoform 2 lacks transferase activity, but it can act as a negative regulator of isoform 1. Expression of UGT1A10 is regulated both epigenetically and transcriptionally in response to oxidative stress and aryl hydrocarbon receptor agonists. Clone 20H1 is highly specific for UGT1A10 that can also detect differentially glycosylated forms of UGT1A10. (Ref.: Oda, S., et al. (2017). Drug Metab. Dispos. 45:1027-1034).

Spécificité

Clone 20H1 specifically detects human UDP-glucuronosyltransferase 1-10. It targets an epitope with in 32 amino acids from the N-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 32 amino acids from the N-terminal region of human UDP-glucuronosyltransferase 1-10.

Application

Anti-UGT1A10, clone 20H1, Cat. No. MABS2070, is a mouse monoclonal antibody that detects UDP-glucuronosyltransferase 1-10 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected UGT1A10 in Western Blotting applications (Oda, S., et. al. (2017). Drug Metab Dispos. 45(9):1027-1034).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected UGT1A10 in Immunohistochemistry applications (Oda, S., et. al. (2017). Drug Metab Dispos. 45(9):1027-1034).

Qualité

Evaluated by Immunohistochemistry (Paraffin) in human small intestine tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected UGT1A10 in human small intestine tissue sections.

Description de la cible

59.81 kDa calculated.

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.