MABS1300

Anti-asparagine synthetase Antibody, clone Z5808

clone Z5808, from mouse

• Synonyme(s) : Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: Z5808, monoclonal
• Espèces réactives: human
• Technique(s): ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001664
• Numéro d'accès UniProt: P08243
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ASNS(440)

Description

Description générale

Asparagine synthetase [glutamine-hydrolyzing] (UniProt: P08243; also known as EC:6.3.5.4, Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase) is encoded by the ASNS (also known as TS11) gene (Gene ID: 440) in human. Asparagine synthetase catalyzes the biosynthesis of L-asparagine from L-aspartate in an ATP-dependent manner. It contains one asparagine synthetase domain and one glutamine amidotransferase type-2 domain. ASNS transcription is enhanced by amino acid deprivation via a signal transduction pathway that senses amino acid availability. Mutations in ASNS gene are reported to result in asparagine synthetase deficiency that is characterized by microcephaly, severely delayed psychomotor development, progressive encephalopathy, and hyperekplexic activity. There has been much interest in the development of asparagine synthetase inhibitors for treatment of asparaginase-resistance leukemias and levels of asparagine synthetase have been considered as a potential biomarker in ovarian cancers.

Spécificité

Clone Z5808 detects Asparagine synthetase in human creast cancer, human tonsil, and human uterus. It targets an epitope within 50 amino acids from the N-terminal end.

Immunogène

N-asparagine synthetase-budded virus.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected asparagine synthetase in human tonsil and human uterus tissues.

Immunocytochemistry Analysis: A representative lot detected asparagine synthetase in Immunocytochemistry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

Flow Cytometry Analysis: A representative lot detected asparagine synthetase in Flow Cytometry applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

ELISA Analysis: A representative lot detected asparagine synthetase in ELISA applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

Western Blotting Analysis: A representative lot detected asparagine synthetase in Western Blotting applications (Kusano-Arai, O., et. al. (2012). Hybridoma (Larchmt). 31(5):325-32).

This mouse monoclonal Anti-asparagine synthetase, clone Z5808, Cat. No. MABS1300 has been tested for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting for the detection of human Asparagine Synthetase.

Qualité

Evaluated by Immunohistochemistry in human breast cancer tissues.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected asparagine synthetase in human breast cancer tissue.

Description de la cible

64.37 kDa calculated.

Forme physique

Format: Purified

Purified mouse monoclonal antibody IgG2a in PBS with 0.01% sodium azide.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable