MABN2302

Anti-ARHGAP11B Antibody, clone 3758-A37-5

• Synonyme(s) : Inactive Rho GTPase-activating protein 11B, Rho-type GTPase-activating protein 11B
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3758-A37-5, monoclonal
• Poids mol.: calculated mol wt 30.25 kDa; observed mol wt ~30 kDa
• Produit purifié par: using protein G
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μL
• Technique(s): immunofluorescence: suitable; western blot: suitable
• Isotype: IgG1κ
• Séquence de l'épitope: C-terminal
• Numéro d'accès Protein ID: NP_001034930
• Numéro d'accès UniProt: Q3KRB8
• Température de stockage: 2-8°C
• Informations sur le gène: human ... ARHGAP11B(89839)

Description

Spécificité

Clone 3758-A37-5 is a mouse monoclonal antibody that detects Rho-type GTPase-activating protein 11B (ARHGAP11B). It targets an epitope within 21 amino acids from the C-terminal region.

Immunogène

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Rho-type GTPase-activating protein 11B (ARHGAP11B).

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from COS-7 cells transfected with human ARHGAP11B.

Western Blotting Analysis: A 1:500 dilution of this antibody detected ARHGAP11B in lysate from COS-7 cells transfected with human ARHGAP11B, but not in lysate from wild-type COS-7 cells.

Tested Applications

Immunofluorescence Analysis: A representative lot detected ARHGAP11B in Immunofluorescence application (Namba, T., et al. (2020). Neuron. 105(5):867-881.e9; Heide, M., et al. (2020). Science. 369(6503):546-550).

Western Blotting Analysis: A representative lot detected ARHGAP11B in Western Blotting applications (Namba, T., et al. (2020). Neuron. 105(5):867-881.e9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Description de la cible

Inactive Rho GTPase-activating protein 11B (UniProt: Q3KRB8; also known as Rho-type GTPase-activating protein 11B) is encoded by the ARHGAP11B (also known as FAM7B1) gene (Gene ID: 89839) in human. ARHGAP11B is a mitochondrial matrix protein that is specifically expressed in radial glia cells of cortical neural stem and progenitor cells during neocortex development. It is preferentially expressed in apical and basal radial glia and is not detected in cortical neurons and cortical plate. It promotes amplification of basal progenitors in the subventricular zone, producing more neurons during fetal corticogenesis, thereby playing a key role in neocortex expansion. It promotes basal progenitor cell proliferation by inhibiting the mitochondrial permeability transition pore (mPTP). It delays the opening of the mPTP via interaction with ADP:ATP translocase, thereby increasing mitochondrial Ca2+ concentration and inducing glutamine catabolism, which is required for basal progenitor proliferation. ARHGAP11B does not exhibit GTPase activator activity and this lack of GTPase activator activity is essential for amplification of basal progenitors during neocortex development. (Ref.: Namba, T., et al. (2020). Neuron. 105(5); 867-881; Florio, M., et al. (2015). Science. 347(6229); 1465-1470).

Forme physique

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable