MABF3174
Anti-IL-17A Antibody, clone 6F9.2.12.1.5
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
6F9.2.12.1.5, monoclonal
Poids mol.
calculated mol wt 17.5 kDa
observed mol wt ~30 kDa
Produit purifié par
using protein G
Espèces réactives
human
Conditionnement
antibody small pack of 100 μg
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Isotype
IgG1κ
Séquence de l'épitope
Unknown
Numéro d'accès Protein ID
Numéro d'accès UniProt
Conditions d'expédition
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... IL17A(7314)
Catégories apparentées
Description générale
Interleukin-17A (UniProt: Q16552; also known as IL-17, IL-17A, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8) is encoded by the IL17A (also known as CTLA8, IL17) gene (Gene ID: 3605) in human. IL-17A is a proinflammatory cytokine secreted by activated CD4+ Th17 cells. It is synthesized with a signal peptide (aa 1-23) that is subsequently cleaved off to produce the mature protein. Its levels are up-regulated by IL23A-IL12B. IL-17 is reported to play an important role in multiple biological processes, including the induction of IL-6, IL-8, G-CSF, and PGE2 production in epithelial, endothelial or fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kB and co-stimulation of T cell proliferation. It is also involved in antimicrobial host defense and maintenance of tissue integrity. Its actions are mediated via interaction with IL-17RA and IL-17RC heterodimeric receptor complex, triggering homotypic interaction of these receptors with (TRAF3 Interacting Protein 2 (TRAF3IP2) adapter. This leads to downstream TRAF6-mediated activation of NF-kB and MAP kinase pathways resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides, and matrix metalloproteinases. IL-17A also plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. In synergy with IL-17F, it is shown to mediate the production of antimicrobial b-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, which limits the entry of microbes through the epithelial barriers. (Ref.: Boisson, B., et al. (2013). Immunity. 39(4); 676-686; Wright, J.F., et al. (2008). J. Immunol. 181(4); 2799-2805).
Spécificité
Clone 6F9.2.12.1.5 is a mouse monoclonal antibody that specifically detects human Interleukin-17A.
Immunogène
A recombinant fragment corresponding to amino acids 19 to 155 of human IL-17A.
Application
Quality Control Testing
Evaluated by Western Blotting with recombinant human IL-17A.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human IL-17A.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-17A in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting with recombinant human IL-17A.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant human IL-17A.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected IL-17A in human tonsil tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-17A, clone 6F9.2.12.1.5, Cat. No. MABF3174, is a mouse monoclonal antibody that detects IL-17A and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Forme physique
Purified mouse monoclonal antibody IgG1 in PBS with 0.05% sodium azide.
Stockage et stabilité
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Certificats d'analyse (COA)
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