MABE461

Anti-CRTC1 Antibody/TORC-1, clone 5F3.1

clone 5F3.1, from mouse

• Synonyme(s) : CREB-regulated transcription coactivator 1, Mucoepidermoid carcinoma translocated protein 1, Transducer of regulated cAMP response element-binding protein 1, TORC-1, Transducer of CREB protein 1, CRTC1/TORC-1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 5F3.1, monoclonal
• Espèces réactives: mouse, rat, human
• Technique(s): immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_001091952
• Numéro d'accès UniProt: Q6UUV9
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CRTC1(23373)

Description

Description générale

CREB-regulated transcription coactivator 1 (UniProt Q6UUV9; also known as Mucoepidermoid carcinoma translocated protein 1, TORC-1, Transducer of CREB protein 1, Transducer of regulated cAMP response element-binding protein 1) is encoded by the CRTC1 (also known as KIAA0616, MECT1, TORC1, WAMTP1) gene (Gene ID 23373) in human. CRTC1 belongs to a family of glycosylated Ser/Thr-rich phosphoproteins that bind the basic leucine zipper (bZIP) region of Ca2+/cAMP-responsive element-binding protein (CREB) and function as CREB coactivators. CRTCs participate in CREB-mediated transcriptions of both canonical cellular cAMP response elements (CREs) and the non-canonical viral CREs. CRTC1, specifically, is involved in CREB-mediated transcription of various neuronal immediate early genes (IEGs) as well as CREB-dependent hepatitis B virus (HBV) replication.

Spécificité

Expected to react with all three human spliced isoforms

Immunogène

Epitope: Internal (N-terminal half)

GST-tagged recombinant protein corresponding to human CRTC1/TORC-1 sequence within the N-terminal half..

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-CRTC1 Antibody/TORC-1, clone 5F3.1 is validated for use in Western Blotting, Immunohistochemistry, Immunoprecipitation for the detection of CRTC1.

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected CRTC1/TORC-1 in 10 µg of mouse hypothalamus tissue lysate.
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected CRTC1/TORC-1 in human cerebral cortex, rat cerebral cortex, human cerebellum, and rat cerebellum tissue.
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous CRTC1/TORC-1 from mouse hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1, but not CRTC2/TORC-2, from lysates of transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain).
Western Blotting Analysis: A representative lot detected endogenous CRTC1/TORC-1 in human, mouse, and rat hippocampus tissue lysates, as well as exogenously expressed murine CRTC1/TORC-1 in lysates from transfected HEK293 cells (Courtesy of Dr. Carlos A. Saura , Universitat Autònoma de Barcelona, Spain).

Qualité

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CRTC1/TORC-1 in 10 µg of mouse cerebellum tissue lysate.

Description de la cible

~68 kDa observed

Forme physique

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable