MABE1073
Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7
clone 21A7, from mouse
Synonyme(s) :
Structural maintenance of chromosomes protein 3, Lys105/106 acetylated, Bamacan, Lys105/106 acetylated, Basement membrane-associated chondroitin proteoglycan, Lys105/106 acetylated, Chondroitin sulfate proteoglycan 6, Lys105/106 acetylated, Chromosome-as
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
21A7, monoclonal
Espèces réactives
human
Réactivité de l'espèce (prédite par homologie)
nonhuman primates (based on 100% sequence homology), Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
Technique(s)
ChIP: suitable (ChIP-seq)
ELISA: suitable
western blot: suitable
Isotype
IgG2bκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
acetylation (Lys105/Lys106)
Informations sur le gène
human ... SMC3(9126)
Description générale
Structural maintenance of chromosomes protein 3 (UniProt Q9UQE7; also known as Bamacan, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP, SMC protein 3, SMC-3) is encoded by the SMC3 (also known as BAM, BMH, CDLS3, CSPG6, SMC3L1) gene (Gene ID 9126) in human. Cohesion is a nuclear tripartite complex composed of Rad21 and two structural maintenance of chromosomes proteins, SMC1 and SMC3. Cohesin plays an important role in sister chromatid cohesion, as well as in DNA repair and gene expression regulation. To establish sister chromatid cohesion during S phase, SMC3 is acetylated (on K105 and K106 in human) by two cohesin acetyltransferases (CoATs), ESCO1 and ESCO2, that differ in their N-terminal domains and expression during development and across the cell cycle. ChIP-seq analysis reveals that ESCO1 is recruited by cohesin to over 11,000 chromatin sites occupied by cohesin and CTCF that mediate long-range chromatin interactions and regulate transcription globally. On ther other hand, ESCO2 is infrequently enriched at sites targeted by REST/NRSF (RE1-silencing transcription factor/neuron-restrictive silencer factor) that represses transcription of neuron-specific genes. SMC3 acetylation is thought to induce conformational changes of the dimeric hinge structure during S phase and to allow dimer opening and loading of DNA.
Spécificité
Clone 21A7 bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Immunogène
KLH-conjugated linear peptide corresponding to the target region sequence of human/mouse/rat SMC3 containing acetylated Lys105 and Lys106.
Application
Anti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting.
ELISA Analysis: A representative lot bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected enhanced SMC3 occupancy at target chromatin sites in HDAC8-null HCT116 cells than wild-type HCT-116 cells (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
ChIP-seq Analysis: A representative lot detected SMC3 chromosome 11 enrichment sites, including 81% of Rad21 target sites and 70% of Esco1 target sites, by ChIP-seq (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Western Blotting Analysis: A representative lot detected SMC3 Lys105/106 acetylation in HeLa cells. ESCO1 or ESCO2 depletion by shRNA treatment reduced SMC3 Lys105/106 acetylation level, while dual ESCO1/2 depletion resulted in most profound SMC3 Lys105/106 acetylation reduction (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected enhanced SMC3 occupancy at target chromatin sites in HDAC8-null HCT116 cells than wild-type HCT-116 cells (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
ChIP-seq Analysis: A representative lot detected SMC3 chromosome 11 enrichment sites, including 81% of Rad21 target sites and 70% of Esco1 target sites, by ChIP-seq (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Western Blotting Analysis: A representative lot detected SMC3 Lys105/106 acetylation in HeLa cells. ESCO1 or ESCO2 depletion by shRNA treatment reduced SMC3 Lys105/106 acetylation level, while dual ESCO1/2 depletion resulted in most profound SMC3 Lys105/106 acetylation reduction (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Qualité
Evaluated by Western Blotting in human retinal epithelial pigment (REP) cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a reduced SMC3 Lys105/106 acetylation in 10 µg of lysate from N-acetyltransferase ESCO1-knockout human retinal epithelial pigment (REP) cells than in lysate from wild-type REP cells.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a reduced SMC3 Lys105/106 acetylation in 10 µg of lysate from N-acetyltransferase ESCO1-knockout human retinal epithelial pigment (REP) cells than in lysate from wild-type REP cells.
Description de la cible
~160 kDa observed. 142.0/141.5/141.6/138.4/140.7 kDa (bovine/human/mouse/rat/xenopus) calculated. Uncharacterized bands may be observed in some lysate(s).
Forme physique
Format: Purified
Protein G purified.
Purified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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Chunlong Zhao et al.
Journal of medicinal chemistry, 67(16), 14016-14039 (2024-08-02)
HDAC8 can mediate signals by using its enzymatic or nonenzymatic functions, which are expected to be critical for various types of cancer. Herein, we employed proteolysis targeting chimera (PROTAC) technology to target the enzymatic as well as the nonenzymatic functions
Tasha B Toro et al.
PloS one, 18(9), e0291779-e0291779 (2023-09-18)
Acetylation of lysine residues is an important and common post-translational regulatory mechanism occurring on thousands of non-histone proteins. Lysine deacetylases (KDACs or HDACs) are a family of enzymes responsible for removing acetylation. To identify the biological mechanisms regulated by individual
Jiaxin Zhang et al.
Nucleic acids research, 51(10), 4760-4773 (2023-03-14)
Besides entrapping sister chromatids, cohesin drives other high-order chromosomal structural dynamics like looping, compartmentalization and condensation. ESCO2 acetylates a subset of cohesin so that cohesion must be established and only be established between nascent sister chromatids. How this process is
Nicole L Arruda et al.
Epigenetics & chromatin, 15(1), 30-30 (2022-08-20)
Cohesin is an important structural regulator of the genome, regulating both three-dimensional genome organization and gene expression. The core cohesin trimer interacts with various HEAT repeat accessory subunits, yielding cohesin complexes of distinct compositions and potentially distinct functions. The roles
Ayad A Al-Hamashi et al.
Bioorganic chemistry, 116, 105297-105297 (2021-09-13)
Despite the advances in treatment strategies, cancer is still the second leading cause of death in the USA. A majority of the currently used cancer drugs have limitations in their clinical use due to poor selectivity, toxic side effects and
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