HTS171RTA
Ready-to-Assay sst 3 Somatostatin Family Receptor Frozen Cells
Human
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41106514
eCl@ss :
32011203
Nomenclature NACRES :
NA.81
Produits recommandés
product name
Ready-to-Assay sst 3 Somatostatin Family Receptor Frozen Cells,
Source biologique
human
Niveau de qualité
Fabricant/nom de marque
Ready-to-Assay
Technique(s)
calcium flux assay: suitable
Numéro d'accès NCBI
Méthode de détection
fluorometric
Description générale
Millipore′s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.
Somatostatin is a 14 or 28 amino acid regulatory peptide that inhibits hormone secretion from the pituitary, pancreas, and other endocrine sites. A family of 6 GPCRs, sst1, sst2A, sst2B, sst3, sst4 and sst5, mediate the biological activity of somatostatins. The somatostatin receptors couple to Gi to inhibit cAMP production, and also increase MAP kinase signalling. Several tumors have been shown to overexpress somatostatin receptors, and binding of somatostatin to these tumor cells stimulates or inhibits proliferation, depending on the receptor subtypes expressed (Olias et al., 2004). However, sst3 appears to promote apoptosis, and expression of sst3 was found to be lower in gastric cancer cells than in normal gastric mucosa, in proportion to susceptibility to apoptosis induced by somatostatin analogs (Sharma et al., 1996; Hu et al., 2004). In addition, sst3 is expressed in smooth muscle cells and mediates gastrointestinal contractions. Millipore′s cloned human sst3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant sst3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at sst3.
Somatostatin is a 14 or 28 amino acid regulatory peptide that inhibits hormone secretion from the pituitary, pancreas, and other endocrine sites. A family of 6 GPCRs, sst1, sst2A, sst2B, sst3, sst4 and sst5, mediate the biological activity of somatostatins. The somatostatin receptors couple to Gi to inhibit cAMP production, and also increase MAP kinase signalling. Several tumors have been shown to overexpress somatostatin receptors, and binding of somatostatin to these tumor cells stimulates or inhibits proliferation, depending on the receptor subtypes expressed (Olias et al., 2004). However, sst3 appears to promote apoptosis, and expression of sst3 was found to be lower in gastric cancer cells than in normal gastric mucosa, in proportion to susceptibility to apoptosis induced by somatostatin analogs (Sharma et al., 1996; Hu et al., 2004). In addition, sst3 is expressed in smooth muscle cells and mediates gastrointestinal contractions. Millipore′s cloned human sst3-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant sst3 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists, antagonists and modulators at sst3.
Description de la lignée cellulaire
- GPCR Cell Lines
- Host cells: Chem-1
Application
Human sst3 GPCR frozen cells for Calcium Flux FLIPR Assays.
Actions biochimiques/physiologiques
- GPCR Class: A
- Protein Target: sst3
- Target Sub-Family: Somatostatin
Composants
Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial. Fifty (50) mL of Media Component.
Clause de non-responsabilité
This product contains genetically modified organisms (GMO).
Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively.
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique