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HSP1MAG-63K

Millipore

MILLIPLEX® Human Sepsis Magnetic Bead Panel 1 - Immune Response Multiplex Assay

Inflammation/Immunology Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple sepsis biomarkers in human serum, plasma and cell culture samples.

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About This Item

Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.84

Niveau de qualité

200

Espèces réactives

human

Fabricant/nom de marque

Milliplex®

assay range

accuracy: 111%
(sVCAM-1)
accuracy: 114%
(MIF)
accuracy: 98%
(sFas)
standard curve range: 12-50,000 pg/mL
(sFas, sFasL, and PAI-1)
standard curve range: 61-250,000 pg/mL
(sVCAM-1 and sICAM-1)

Technique(s)

multiplexing: suitable

Méthode de détection

fluorometric (Luminex xMAP)

Conditions d'expédition

wet ice

Description générale

Sepsis or SIRS (systemic inflammatory response syndrome) results from infection, whether bacterial, viral, fungal, or parasitic. According to the CDC, sepsis is the second leading cause of death in non-coronary ICU patients in the U.S. The pathogenesis of sepsis is very complex. Both an innate and acquired immune response to infection interrupts homeostasis with a shift of cytokine expression that increases immunosuppression. In addition, migration of the leukocytes in the inflamed tissues secretes a tissue factor leading to the formation of thrombin, also triggering the release of chemokines and adhesion molecules through endothelial cells. Severe sepsis occurs when hypotension or hypoperfusion to one or more organs leads to organ dysfunction, which, in turn, can cause septic shock, multiple organ dysfunction syndrome (MODS) and death.

MILLIPLEX® Human Sepsis Panel 1 is to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma and cell / tissue culture supernatant samples: sFAS/TNFRSF6, sFasL, sICAM-1, MIF, PAI-1 (total), sVCAM-1. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Immune Response

Application

  • Analytes: MIF, PAI-1 (Total), sFas, sFasL, sICAM-1, sVCAM-1
  • Recommended Sample type: Serum, plasma, tissue culture, or homogenates
  • Recommended Sample dilution: 1:10 for serum/plasma
  • Assay Run Time: Overnight
  • Research Category: Inflammation & Immunology

Caractéristiques et avantages

Design your multiplex kit by choosing available analytes within this panel.

Autres remarques

Sensitivity: Refer to kit protocol for individual sensitivities.
Standard Curve Range: Refer to QC analysis sheet for exact concentration (MIF).

Informations légales

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Pictogrammes

Exclamation markEnvironment
GHS07,GHS09

Mention d'avertissement

Warning

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

10 - Combustible liquids

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Sigita Kazune et al.
Critical care (London, England), 23(1), 311-311 (2019-09-13)
In patients with septic shock, the skin is often chosen for the evaluation of peripheral perfusion and oxygenation. Changes in skin microcirculatory vessel oxygen saturation and relative hemoglobin concentration can be described using a mottling score or captured with hyperspectral
Dai Kimura et al.
Frontiers in pediatrics, 4, 31-31 (2016-04-12)
Lung injury activates multiple pro-inflammatory pathways, including neutrophils, epithelial, and endothelial injury, and coagulation factors leading to acute respiratory distress syndrome (ARDS). Low-dose methylprednisolone therapy (MPT) improved oxygenation and ventilation in early pediatric ARDS without altering duration of mechanical ventilation
Miša Korva et al.
Viruses, 11(8) (2019-07-31)
Hemorrhagic fever with renal syndrome (HFRS) and Crimean-Congo hemorrhagic fever (CCHF) are important viral hemorrhagic fevers (VHF), especially in the Balkan region. Infections with Dobrava or Puumala orthohantavirus and Crimean-Congo hemorrhagic fever orthonairovirus can vary from a mild, nonspecific febrile
Sonia Y Velásquez et al.
The Journal of biological chemistry, 291(25), 12960-12977 (2016-04-30)
Natural killer (NK) cells induce apoptosis in infected and transformed cells and are important producers of immunoregulatory cytokines. Therefore, they operate under low oxygen conditions (hypoxia) in inflammatory and tumor environments. In vitro studies of NK cells are, however, commonly

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