ECM610
PAI Activity Assay
The PAI Activity Assay Kit provides a quick, efficient & sensitive system for evaluation of inhibition of uPA activity by PAI & for screening of uPA inhibitors.
About This Item
Produits recommandés
Niveau de qualité
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
activity assay: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Informations sur le gène
human ... PLAU(5328)
Description générale
The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.
The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.
Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E.Coli-expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.
The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Test Principle:
The CHEMICON PAI Activity Assay Kit utilizes a chromogenic substrate, which is cleaved by active uPA, detectable by its Optical Density at 405nm (OD405). Addition of PAI sample blocks the cleavage of substrate by uPA.
Application:
The CHEMICON PAI Assay Kit is ideal for measurement of PAI inhibitory activity toward uPA in purified preparations and cell culture, as well as in serum where pathological conditions such as sepsis exist. The assay is also useful for screening inhibitors of uPA.
Each CHEMICON PAI Activity Assay Kit contains sufficient reagents for the evaluation of 96 samples, including uPA standard from human urine and E. coli expressed recombinant protein corresponding to amino acids 24 to 427 of human PAI-1 (mature form) as positive control. PAI-1 positive control contains a mixture of the active and latent forms, with >45% of active form. Duplicate or triplicate samples are suggested.
The CHEMICON PAI Activity Assay Kit is intended for research use only; not for diagnostic or therapeutic applications.
Application
Cell Structure
Conditionnement
Composants
Chromogenic Substrate: (Part No. 90545) One 5 mg bottle of Tripeptide with pNA group.
Assay Buffer, 10X: (Part No. 90091) One 5 mL bottle.
PAI-1 Positive Control: (Part No. 90093) One 400 μL vial containing 20 μg of purified PAI-1 with 0.1% BSA.
Stockage et stabilité
Informations légales
Clause de non-responsabilité
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
10 - Combustible liquids
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