CP11L

Anti-Integrin α₃ Mouse mAb (P1B5)

lyophilized, clone P1B5, Calbiochem^®

• Code UNSPSC : 12352203

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: P1B5, monoclonal
• Forme: lyophilized
• Ne contient pas: preservative
• Espèces réactives: human
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Isotype: IgG1
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... ITGA3(3675)

Description

Description générale

Purified mouse monoclonal antibody generated by immunizing RBF/Dn mice with the specified immunogen and fusing splenocytes with NS-1/FOX-NY mouse myeloma cells (see application references). Recognizes the α3 integrin subunit.

Recognizes the α₃-subunit of the α₃/β₁ integrin complex protein in human mononuclear cells.

This Anti-Integrin α₃ Mouse mAb (P1B5) is validated for use in Cell Inhibition Assays, FC, IF, IP for the detection of Integrin α₃.

Immunogène

Human

human mononuclear cells

Application

Cell Inhibition Assays (1-5 µg/ml, see application references)

Flow Cytometry (1-5 µg/ml)

Immunoblotting (not recommended)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Paraffin Sections (not recommended)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage (with azide) or aliquot and freeze (-20°C) for short-term storage.

Remarque sur l'analyse

Positive Control
A2058 cells

Autres remarques

Albelda, S.M. and Buck, C.A. 1990. FASEB J.4, 2868.
Cheresh, D.A. et al. 1990. Cell57, 59.
Krissansen, G.W. et al. 1990. J. Biol. Chem.265, 823.
Vogel, B, et al. 1990. J. Biol. Chem.265, 5934.
Wayner, E.A. et al.1988 J. Cell Biol.107, 1881.
Buck, C.A. and Horwitz, A.F. 1987. Rev. Cell Biol.3, 179.
Hynes, R.O. 1987 Cell48, 549.
Wayner, E.A. and Carter, W.G., 1987. J. Cell Biol.105, 1873.

Will partially inhibit cell-cell adhesion of E1L-8 cells. Antibody should be titrated for optimal results in individual systems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable