ABE1878

Anti-UPF3A

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Poids mol.: calculated mol wt 54.70 kDa; observed mol wt ~54 kDa
• Espèces réactives: human
• Conditionnement: antibody small pack of 100 μg
• Technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès UniProt: Q9H1J1
• Conditions d'expédition: dry ice
• Température de stockage: 2-8°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... UPF3A(65110)

Description

Description générale

Regulator of nonsense transcripts 3A (UniProt: Q9H1J1; also known as Nonsense mRNA reducing factor 3A, Up-frameshift suppressor 3 homolog A, hUpf3, UPF3A) is encoded by the UPF3A (also known as RENT3A, UPF3) gene (Gene ID: 65110) in human. UPF3A acts as an inhibitor of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. It is reported to shuttle between the nucleus and the cytoplasm. It recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. It binds spliced mRNA upstream of exon-exon junctions. Mice lacking UPF3A exhibit hyper NMD and show defects in embryogenesis and gametogenesis. In human three isoforms of UPF3A have been described that are produced by alternative splicing. (Ref.: Shum, EY., et al. (2016). Cell. 165(2); 382-395).

Spécificité

This rabbit polyclonal antibody detects human Regulator of nonsense transcripts 3A. It targets an epitope within 16 amino acids from the C-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal half of human Regulator of nonsense transcripts 3A (UPF3A).

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected UPF3A in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected UPF3A in human fetal brain tissue lysate.

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected UPF3A in human testis and human colon tissue sections.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected UPF3A in lysate from HeLa cells over expressing UPF3A. (Courtesy of Matthew Kim & Dr. Wilkinson Miles at UCSD).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-UPF3A, Cat. No. ABE1878, is a rabbit polyclonal antibody that detects Regulator of nonsense transcripts 3A (UPF3A) and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Forme physique

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at +2°C to +8°C from date of receipt.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable