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AB9220

Sigma-Aldrich

Anti-Nanog Antibody

serum, Chemicon®

Synonyme(s) :

Anti-NANOG/STM1, Anti-STM1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

serum

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

mouse, human

Fabricant/nom de marque

Chemicon®

Technique(s)

flow cytometry: suitable
western blot: suitable

Entrée

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... NANOG(79923)
mouse ... Nanog(71950)

Description générale

NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).

Spécificité

Recognizes Nanog, human.

Immunogène

Synthetic peptide from human Nanog.

Application

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.
Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.

Optimal working dilutions must be determined by the end user.

Description de la cible

39 kDa (MW 34 kDa)

Forme physique

Rabbit Serum. Liquid.
Unpurified

Stockage et stabilité

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Whole cell lysate from mouse embryonic stem cells

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

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Arlette A Haidar-Montes et al.
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