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53702

Millipore

Pronase® Protease, Streptomyces griseus

Synonyme(s) :

Pronase® powder

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.54

Forme

lyophilized

Niveau de qualité

Activité spécifique

≥45,000 proteolytic units/g dry wt

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
desiccated (hygroscopic)

Solubilité

water: 10 mg/mL

Conditions d'expédition

ambient

Température de stockage

2-8°C

Description générale

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but it is stable over a wide pH and temperature range. Has an optimal pH of 7.5. Note: 1 KU = 1000 units.
A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.
Note: 1 KU = 1000 units.

Avertissement

Toxicity: Harmful (C)

Définition de l'unité

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Forme physique

Lyophilized from a solution containing calcium acetate as a stabilizer.

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for 24 h at 4°C.

Autres remarques

Narahashi, Y., et al. 1968. J. Biochem. 64, 427.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazardExclamation mark

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2


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T E Davis et al.
Infection and immunity, 15(3), 978-987 (1977-03-01)
Enzymes capable of hydrolyzing cell walls of Blastomyces dermatitidis and chemotypes I and II of Histoplasma capsulatum were prepared in the laboratory or obtained from commercial sources. They included chitinases, beta-1,3-glucanases, beta-1,6-glucanase, and Pronase. Monosaccharides and disaccharides of glucose released
Nicole M Templeman et al.
Developmental cell, 54(1), 92-105 (2020-06-17)
Evolutionarily conserved signaling pathways are crucial for adjusting growth, reproduction, and cell maintenance in response to altered environmental conditions or energy balance. However, we have an incomplete understanding of the signaling networks and mechanistic changes that coordinate physiological changes across
Yifei Jiang et al.
Anesthesiology, 125(6), 1159-1170 (2016-09-23)
Exposure to isoflurane increases apoptosis among postnatally generated hippocampal dentate granule cells. These neurons play important roles in cognition and behavior, so their permanent loss could explain deficits after surgical procedures. To determine whether developmental anesthesia exposure leads to persistent
Jun Sunayama et al.
Stem cells (Dayton, Ohio), 28(11), 1930-1939 (2010-09-22)
The molecular signaling pathways orchestrating the biology of cancer stem-like cells (CSLCs), including glioblastoma, remain to be elucidated. We investigated in this study the role of the MEK/extracellular signal-regulated kinase (ERK) pathway in the control of self-renewal and tumorigenicity of
Kenya Terabe et al.
The Journal of biological chemistry, 294(47), 17799-17817 (2019-10-18)
Hyaluronan is a critical component of articular cartilage and partially helps retain aggrecan within the extracellular matrix of this tissue. During osteoarthritis, hyaluronan and aggrecan loss are an early sign of tissue damage. However, our recent attempts to mimic hyaluronan

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