492027-M

NRG1 Isoform SMDF, Human, Recombinant

• Synonyme(s) : Heregulin-1
• Code UNSPSC : 12352203

Propriétés

• Pureté: >95% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Impuretés: ≤1.0 EU/μg protein Endotoxin
• Température de stockage: −20°C

Description

Description générale

Recombinant, human neuregulin 1 (NRG1), isoform SMDF (sensory and motor neuron-derived factor), consisting of amino acids 1-263, 117-296, and 162-296, each fused at the C-terminus to a His•Tag sequence and expressed in S. frugiperda Sf21 cells using a baculovirus expression system. The neuregulin (also known as heregulin) family of cytokines is comprised of multiple secreted or membrane-bound isorforms that are produced from the single heregulin gene by alternative splicing and/or usage of different promoters. NRG1 isoform SMDF is a type III isoform with a C-terminal EGF-like domain that interacts with the erbB family of tyrosine kinase receptors. NRG1 isoform SMDF expression has been found to be restricted to the nervous system and is believed to play an important role in the development of the nervous system.

Actions biochimiques/physiologiques

ED₅₀ = 1-5 ng/ml as measured in a serum-free proliferation assay using MCF-7 human breast adenocarcinoma cells.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstituion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Autres remarques

Meyer, D. et al. 1997. Development124, 3575.
Yarden, Y. and D. Wen 1994. in Guidebook to Cytokinesand Their Receptors, N.A. Nicola, Ed., Oxford University Press, p. 146.
Karey, K.P., et al. 1988. Cancer Res.48, 4083.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3